南方农业学报2025，Vol.56Issue(8)：2519-2533,15.DOI:10.3969/j.issn.2095-1191.2025.08.013
江南地区牡丹引种综合性评价及孢粉学聚类分析
Comprehensive evaluation and palynological cluster analysis of introduced peony cultivars in Jiangnan region
摘要
Abstract
[Objective]This study aimed to conduct a comprehensive evaluation of introduced peony cultivars in Jiang-nan region and analyze the genetic relationships among the introduced cultivars,so as to provide a theoretical basis for breeding peony cultivars adapted to local conditions.[Method]Field investigations on 33 introduced peony cultivars in the Jiangnan region were conducted using the analytic hierarchy process(AHP).The 33 introduced peony cultivars were evaluated from the perspective of ornamental value,growth traits,ecological adaptability and so on to establish a compre-hensive evaluation system for screening peony cultivars of high-adaptability.Concomitantly,pollen morphology was ob-served,and cluster analysis was performed to determine genetic relationships among the cultivars.[Result]Based on the characteristics of peonies,a four-tiered hierarchical model was established,comprising the target layer,constraint layer,criterion layer,and base layer.By incorporating ornamental value,growth traits,and ecological adaptability as compre-hensive evaluation factors,an evaluation model for introduced peonies.In the criterion layer,the weight value of growth traits was the highest(0.5813),while in the factor layer,the weight value of branch number was the highest(0.4960).The evaluated cultivars were classified into four grades based on comprehensive assessment scores:GradeⅠ,Grade Ⅱ,Grade Ⅲ,and Grade Ⅳ.Three cultivars were rated as excellent cultivars in GradeⅠ:Xiangxiuqiu,Daojin and Linfeng;nine cultivars were rated as good cultivars in Grade Ⅱ;eighteen were rated as average cultivars in Grade Ⅲ;and three were rated as poor cultivars in Grade Ⅳ:Haihuang,Taiyang and Xinyungu.Scanning electron microscopy revealed that the pollen of 33 introduced peony cultivars occurred as single grains,most of which were prolate spheroidal(polar axis/equa-torial axis>2.00).In polar view,the pollen grains appeared trilobate-rounded,with three colporate germinal apertures,and the exine ornamentation was predominantly foveolate-reticulate.Cluster analysis based on quantitative indicators of pollen morphological characteristics revealed significant diversity in exine ornamentation patterns among the introduced tree peony cultivars.At a Euclidean genetic distance threshold of 1.5,the cultivars were classified into five groups:GroupⅠwith 23 cultivars,Group Ⅱ with 2 cultivars,Group Ⅲ with 6 cultivars,and Groups IV and V each with one cultivar.At a Euclidean genetic distance threshold of 5.0,the cultivars were classified into two groups:Group A with 31 cultivars(including GroupⅠ-Ⅲ),and Cluster B with 2 cultivars(including Group Ⅳ-Ⅴ).[Conclusion]When introducing peony in Jiangnan region,the priority is considering growth traits,followed by adaptability and ornamental value.GradeⅠculti-vars,Xiang Xiuqiu,Dao Jin and Lin Feng,are recommended for large-scale introduction as principal varieties.Grade Ⅱcultivars with relatively good performance can be introduced based on demand,while Grade Ⅲ and Ⅳ cultivars with un-satisfactory performance and are not recommended for introduction.Among the introduced peony cultivars,Haihuang and Rimu are distantly related to the other cultivars,whereas Shouanhong and Fengdanlan are closely related.关键词
牡丹/引种/综合评价体系/层次分析法/花粉形态/聚类分析Key words
peony/introduction/comprehensive evaluation system/analytic hierarchy process/pollen morpho-logy/cluster analysis分类
农业科技引用本文复制引用
鹿泽云,张俊丽,乔木,俞伟鹏,王馨怡,朱柳玲,王千千,陈霞,朱向涛..江南地区牡丹引种综合性评价及孢粉学聚类分析[J].南方农业学报,2025,56(8):2519-2533,15.基金项目
国家自然科学基金项目(32372742,32301643) （32372742,32301643）
浙江省基础公益研究项目(LGN22C160006,RC2023B07) National Natural Science Foundation of China(32372742,32301643) （LGN22C160006,RC2023B07）
Zhejiang Basic Public Welfare Research Project(LGN22C160006,RC2023B07) （LGN22C160006,RC2023B07）
