贵州农业科学2025，Vol.53Issue(10)：35-44,10.DOI:10.3969/j.issn.1001-3601.2025.10.005
基于非靶向代谢组学的红曲菌发酵夏秋茶低分子成分分析
Analysis on Low Molecular Components of Summer-autumn Tea Fermented by Monascus Based on Untargeted Metabolomics
摘要
Abstract
[Objective]The effect of Monascus fermentation on low-molecular components of summer-autumn tea was investigated to provide the scientific basis for developing its characteristic functional components and metabolic pathways.[Method]The untargeted metabolomics of summer-autumn tea fermented by Monascus was analyzed by gas chromatography-mass spectrometry(GC-MS).The differential metabolites between the fermented and unfermented summer and autumn tea were screened.The differential metabolites were analyzed by KEGG metabolic pathway to explore the important metabolic pathways.[Result]There were 30 unique metabolites in summer-autumn tea fermented by Monascus,and 73 unique metabolites in unfermented summer-autumn tea.A total of 59 differential metabolites were screened,25 differential metabolites including taxifolin and caffeine were significant up-regulation.34 differential metabolites were significant down-regulation.The top 20 differential metabolites in summer-autumn tea fermented by Monascus,were divided into 7 kinds of substances of sugars,alkaloids,pyridines,alcohols,acids,lipids and flavonoids.9 metabolites and 11 metabolites were significant up-regulation and down-regulation respectively.All sugars substances were down-regulation almost,acids substances were significant down-regulation,and mannitol,erythritol and D-mannitol in alcohols were significant up-regulation.67 functional pathways were annotated by KEGG metabolic pathway analysis.10,38,7,1,8 and 3 pathways were related to the biological system,metabolism,human diseases,genetic information processing,environmental information processing and cellular processes,respectively.The nonalcoholic fatty liver,type Ⅱ diabetes mellitus and ABC transporter pathway were significant down-regulation.[Conclusion]The taxifolin and caffeine are the unique small molecular functional components of summer-autumn tea fermented by Monascus and the nonalcoholic fatty liver,type Ⅱ diabetes mellitus and ABC transporter pathway are the important differential pathways of summer-autumn tea fermented by Monascus,which suggests that summer-autumn tea fermented by Monascus has an application value in improving metabolic diseases.关键词
红曲菌/夏秋茶/气相色谱-质谱/代谢组学/非靶向/差异代谢物/代谢通路Key words
Monascus/summer-autumn tea/gas chromatography-mass spectrometry/metabonomics/untargeted/differential metabolites/metabolism pathway分类
轻工纺织引用本文复制引用
贺圣凌,周罗娜,宋诗颖,赵兴丽,谭玉梅..基于非靶向代谢组学的红曲菌发酵夏秋茶低分子成分分析[J].贵州农业科学,2025,53(10):35-44,10.基金项目
贵州省科技计划项目"人工接种型金茯黔茶标准化生产应用与推广"(黔科合成果[2025]一般030) （黔科合成果[2025]一般030）
中央引导科技发展专项资金项目"贵州生态黑茶品质风味形成机理及微生物多样性研究"(黔科合中引地[2023]027) （黔科合中引地[2023]027）
