广州医药2025，Vol.56Issue(9)：1165-1174,10.DOI:10.20223/j.cnki.1000-8535.2025.09.002
胆管结扎诱导的梗阻性胆汁淤积对大鼠肝脏肝细胞影响的初步探究
Preliminary study of bile duct ligation induced obstructive cholestasis in rat hepatocytes
摘要
Abstract
Objective To explore the effect of bile duct ligation induced obstructive cholestasis on rat hepatocytes.Methods Ten Lewis rats were randomly divided into control group and cholestasis group,and the cholestasis was induced by bile duct ligation for 2 weeks.The histopathological changes were compared by H&E and aniline blue staining and the liver function was compared by biochemical analysis.Primary hepatocytes were isolated by modified two-step collagenase perfusion,and the expressions of hepatocyte marker genes,cell proliferation marker genes and cholangiocyte marker genes were detected by RT-qPCR.Results Compared with the control group,the liver of the cholestatic group showed obvious disordered histopathology,deposition of fibrous collagen and impaired liver function.Compared with the control group,the primary hepatocytes in the cholestasis group expressed higher cell proliferation-related genes(Ki67,Foxm1,Pcna and HGF)(P＜0.05).Primary hepatocytes in the cholestasis group expressed lower levels of hepatocyte marker genes(Alb,Mrp2,Bsep and Catenin1)(P＜0.05),and higher levels of cholangiocyte marker genes(Ck7,Ck19,Mdr1 and Cftr)(P＜0.05)and higher levels of the hepatic progenitor cell marker genes(Epcam and Sox9)(P＜0.05).Conclusions Cholestasis induces rat hepatocyte plasticity in the transformation into bile duct properties.关键词
胆汁淤积性肝病/肝细胞/胆管细胞/细胞可塑性/胆管结扎Key words
cholestatic liver disease/hepatocyte/cholangiocyte/cell plasticity/bile duct ligation引用本文复制引用
黄宇,宫本大辅,李天洋,李佩霖,翁杰锋,古维立..胆管结扎诱导的梗阻性胆汁淤积对大鼠肝脏肝细胞影响的初步探究[J].广州医药,2025,56(9):1165-1174,10.基金项目
广东省医学科研基金项目(A2024088) （A2024088）
广州市科技计划项目(2024A03J1016) （2024A03J1016）
广州市中医药和中西医结合科技项目(20242A011001) （20242A011001）
