广州医药2025，Vol.56Issue(9)：1238-1244,1295,8.DOI:10.20223/j.cnki.1000-8535.2025.09.011
细胞焦亡现象在大鼠急性心肌梗死无复流模型中的观察分析研究
Observation and analysis of pyroptosis in the no-reflow model after acute myocardial infarction in rats
摘要
Abstract
Objective To establish a rat model of myocardial no-reflow after acute myocardial infarction with ischemia-reperfusion injury and to preliminarily explore the occurrence of pyroptosis in the affected myocardium.Methods Twenty adult male Sprague-Dawley rats(260-320 g)were randomly divided into a control group(n=5)and a surgical group(n=15).In the control group,the coronary artery was encircled with suture but not ligated.In the surgical group,the left anterior descending artery was ligated for 30 minutes,followed by 4 hours of reperfusion to induce the no-reflow model.Evans blue and thioflavin S staining were used to evaluate the normal perfusion area,reperfusion area,and no-reflow area of the myocardium.Histopathological analysis was conducted on myocardial tissues from both groups.Results All rats in the control group survived without evidence of no-reflow or pyroptosis in myocardial tissue.In the surgical group,13 rats survived and showed distinct regions of normal perfusion,13 with reperfusion,and 10 with no-reflow.Pyroptosis was observed in all no-reflow areas(n=10),absent in the normal perfusion zones(n=0),and partially present in the reperfusion zones(n=4).The differences were statistically significant(P＜0.05).Conclusions Pyroptosis predominantly occurs in the no-reflow zones following acute myocardial infarction and ischemia-reperfusion injury in rats.As a region-specific form of programmed cell death,pyroptosis may play an important role in the development of myocardial no-reflow.关键词
无复流/细胞焦亡/急性心肌梗死Key words
no-reflow/pyroptosis/acute myocardial infarction引用本文复制引用
陈泳华,武泓光,李希大,曾军,杨智,苏伟,王巧玲,张昊天,董豪坚..细胞焦亡现象在大鼠急性心肌梗死无复流模型中的观察分析研究[J].广州医药,2025,56(9):1238-1244,1295,8.基金项目
国家重点研发计划(2016YFC1301202) （2016YFC1301202）
广州市中医药和中西医结合科技项目(20222A010002) （20222A010002）
