华西口腔医学杂志2025，Vol.43Issue(5)：636-647,12.DOI:10.7518/hxkq.2025.2024422
芦荟大黄素通过血小板凝血酶蛋白-1-PI3k-Akt通路抑制瘢痕组织纤维化的研究
Aloe-emodin inhibits scar tissue fibrosis through thrombospondin-1-PI3k-Akt pathway
摘要
Abstract
Objective To propose a hypothesis that aloe-emodin may inhibit scar tissue fibrosis through thrombos-pondin-1(THBS1)-PI3K-Akt pathway.Methods By cultivating fibroblasts derived from scar tissue after cleft palate surgery in humans,aloe emodin of different concentrations(10,20,30,40 and 50 μmol/L)was added to the cells which activity was detected.At the same time,transcriptome sequencing was performed on scar tissue and cells,and bioinfor-matics methods were used to explore potential targets and signaling pathways of scar tissue fibrosis.Results Aloe-emo-din had a concentration dependent inhibitory effect on fibroblast proliferation,with the 40 μmol/L concentration group showing the most significant effect.The results of tissue and cell sequencing indicated that differentially expressed genes were significantly enriched in extracellular matrix-recep-tor interaction pathway,and shared a common differential gene which was THBS1.The ORA analysis results indi-cated that differentially expressed genes,including TH-BS1,were significantly enriched in the PI3K-Akt signaling pathway.Conclusion Aloe emodin may inhibit the PI3K-Akt pathway by downregulating THBS1,thereby reducing the proliferation activity of fibroblasts derived from postoper-ative palatal scar tissue.关键词
芦荟大黄素/血小板凝血酶蛋白-1/PI3K-Akt信号通路/纤维化/腭部瘢痕Key words
aloe-emodin/thrombospondin-1/PI3K-Akt signaling pathway/fibrosis/palatal scar分类
医药卫生引用本文复制引用
耿红宝,张星懿,周思玮,李娜,刘佳,苑学微,宁春柳,张旭东,黄威..芦荟大黄素通过血小板凝血酶蛋白-1-PI3k-Akt通路抑制瘢痕组织纤维化的研究[J].华西口腔医学杂志,2025,43(5):636-647,12.基金项目
Medical Science Research Project in Hebei Province(20240510) 河北省医学科学研究课题计划(20240510) （20240510）
