华西口腔医学杂志2025，Vol.43Issue(5)：648-659,12.DOI:10.7518/hxkq.2025.2025004
硅酸钙的缺陷改性及其在口腔抑菌和牙齿再矿化的应用研究
Defect modification of calcium silicate and its application in oral bacteriostasis and tooth remineralization
摘要
Abstract
Objective Calcium silicate(CSO)is mod-ified to give it photothermal antibacterial properties.Its application potential in tooth mineralization and oral anti-bacterial is evaluated.Methods Based on defect-engi-neering modification strategy,a series of CSO-T samples(CSO-300,CSO-400,CSO-500,CSO-600)was obtained by introducing oxygen vacancy into CSO through thermal re-duction using sodium borohydride.The samples were tested using scanning electron microscopy(SEM),X-ray diffrac-tion,X-ray photoelectron spectroscopy,ultraviolet near-infrared absorption spectroscopy,and infrared thermography.The powder samples with the best photothermal performance and the most suitable material concentration(CSO-500,500 μg/mL)were selected for subsequent experiments.High resolution transmission electron microscopy was used to an-alyze the microstructure and morphology of the sample,and MTT assay and Calcein AM/PI live/dead cell staining were used to evaluate the toxicity and compatibility of the sample to human oral keratinocytes.Escherichia coli and Staphylo-coccus aureus were selected for photothermal antibacterial experiments to evaluate their in vitro antibacterial perfor-mance.SEM,energy dispersive spectrometer,and micro Vickers hardness tester were used to evaluate the ability of mate-rials to induce in vitro remineralization of detached teeth.Results Oxygen vacancies changed the crystal type and lat-tice spacing of CaSiO3,broadened the light-absorption range,and gave it a good photothermal conversion ability in re-sponse to near infrared.In vitro experiments showed that the modified CaSiO3 could promote the formation of hydroxy-apatite on the tooth surface,thereby promoting the remineralization of teeth and improving the teeth hardness.Moreover,it had photothermal antibacterial properties and no cytotoxicity.Conclusion Defect-modified black calcium silicate has multiple functions,such as promoting tooth remineralization and photothermal bacteriostatic.When combined with the infrared luminescent toothbrush,it can simply and effectively treat tooth enamel erosion and oral bacteriostatic diseases caused by the excessive consumption of carbonated beverages and other daily bad living habits.This combination is ex-pected to achieve the synergic treatment effect of tooth remineralization and oral bacteriostatic through daily cleaning is expected.关键词
牙齿再矿化/硅酸钙/光热效应/抑菌/氧空位Key words
tooth remineralization/calcium silicate/photothermal effect/bacteriostasis/oxygen vacancy分类
医药卫生引用本文复制引用
胡媛媛,张书颜,张键华,罗红蓉,李运峰,张静,陈显春..硅酸钙的缺陷改性及其在口腔抑菌和牙齿再矿化的应用研究[J].华西口腔医学杂志,2025,43(5):648-659,12.基金项目
National Key Research and Development Program(2022YFB3804500) （2022YFB3804500）
National Natural Science Foun-dation of China(52473312) 国家重点研发计划(2022YFB3804500) （52473312）
国家自然科学基金(52473312) （52473312）
