华西口腔医学杂志2025，Vol.43Issue(5)：660-670,11.DOI:10.7518/hxkq.2025.2024341
母抗十二肽同源物7在头颈鳞状细胞癌的表达及其预后诊断价值
Expression and prognostic value of mothers against decapentaplegic homolog 7 in head and neck squamous cell carcinoma
摘要
Abstract
Objective This study aimed to explore the biological functions and clinical value of mothers against decapentaplegic homolog(SMAD)7 in head and neck squamous cell carcinoma(HNSCC)through bioin-formatics analysis and basic experiments.Methods The expression of SMAD7 in HNSCC in public databases was studied.Western blot was used to detect the expression of SMAD7 in HNSCC cell lines and normal epithelial cells.The SMAD7 highly expressed HNSCC cell line HSC-4 was si-lenced,and CCK-8,Transwell assays,and cell scratch experiments were conducted to study the effect of SMAD7 on the biological functions of HSC-4 cells.HNSCC expression profile data were obtained from UCSC xena,and genes related to SMAD7 were selected for gene ontology and Kyoto encyclopedia of genes and genomes gene enrichment analysis,construction of a co-expression gene interaction network,and screening of related cell signaling pathways.Western blot was used to detect the expression changes of proteins in the related cell signaling pathways in HNSCC cells with si-lenced SMAD7.cBioPortal was utilized to analyze the mutation rate of the SMAD7 gene,and the MethSurv database was used to analyze the methylation level of the SMAD7 gene and its correlation with prognosis.The receiver operating characteristic curve was used to assess the diagnostic value of SMAD7 for HNSCC.TIMER2.0 was used to analyze the correlation between SMAD7 expression and immune cell infiltration.Results SMAD7 was highly expressed in HN-SCC tumor tissues and some cell lines.Silencing the expression of SMAD7 can significantly inhibit the proliferation,mi-gration,and invasion of cancer cells.Silencing SMAD7 can induce the downregulation of vascular cell adhesion mole-cule 1(VCAM-1).The bioinformatics analysis showed that the mutation rate of the SMAD7 gene and the methylation level were significantly correlated with the prognosis of patients with HNSCC.The expression of SMAD7 was related to the level of immune cell infiltration in HNSCC.Conclusion SMAD7 promotes the proliferation,migration,and inva-sion of HNSCC cells by regulating the expression of VCAM-1.It may be a potential tumor biomarker and therapeutic tar-get for HNSCC.关键词
母抗十二肽同源物7/头颈鳞状细胞癌/血管细胞黏附分子1/诊断/免疫治疗Key words
mothers against decapentaplegic homolog 7/head and neck squamous cell carcinoma/vascular cell adhesion molecule 1/diagnosis/immunotherapy分类
医药卫生引用本文复制引用
赵海惠,钟晓娟,黄怡,费伟..母抗十二肽同源物7在头颈鳞状细胞癌的表达及其预后诊断价值[J].华西口腔医学杂志,2025,43(5):660-670,11.基金项目
The Scientific Research Foundation of Sichuan Provincial Academy of Medical Sciences&Sichuan Pro-vincial People's Hospital(2021LZ01) 四川省医学科学院·四川省人民医院院科研基金(20-21LZ01) （2021LZ01）
