华西口腔医学杂志2025，Vol.43Issue(5)：696-710,15.DOI:10.7518/hxkq.2025.2025049
墨旱莲—女贞子治疗牙周炎的潜在成分及作用机制
Mechanism of Eclipta prostrata L-Ligustrum lucidum Ait in the treatment of periodontitis
摘要
Abstract
Objective This study aimed to explore the potential target and molecular mechanism of Eclipta prostrata L-Ligustrum Lucidum Ait(EPL-LLA)in the treatment of periodontitis by using network pharmacology and molecular docking technology,and to explore its biocompatibility,regulatory effects on inflammatory factors,and antioxidant acti-vity through in vitro experiments.Methods The active components and potential targets of EPL-LLA were screened and predicted through a variety of databases,and the in-tersection of EPL-LLA and periodontitis targets was se-lected.The protein interaction network(PPI)was ana-lyzed by the string platform.The Metascape database was used for gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analy-sis.The active ingredients from the top 6 degrees were docked with the core targets,and the results of binding energy were visualized.An in vitro cell model was established to evaluate the biocompatibility,modulation of inflammatory fac-tors,and antioxidative effects of EPL-LLA through cell counting kit-8(CCK-8),quantitative real-time polymerase chain reaction(qRT-PCR)and 2',7'-Dichlorodihydrofluorescein diacetate(DCFH-DA)fluorescent probe assays.Results Screening revealed 13 active components in EPL corresponding to 220 potential targets,10 active components in LLA corresponding to 283 potential targets,and 1 643 periodontitis-related targets,with 91 shared targets among the three.GO analysis of the shared targets yielded 5 271 entries,while KEGG enrichment analysis indicated involvement in 253 signaling pathways.Molecular docking confirmed stable binding between the top 6 active components and core targets.CCK-8 assays demonstrated good biocompatibility of EPL-LLA at concentrations 0.02 mg/mL(P<0.05).qRT-PCR showed that EPL-LLA reduced the mRNA expression of pro-inflammatory factors in macrophages stimulated by Porphy-romonas gingivalis lipopolysaccharide while upregulating anti-inflammatory factor mRNA expression(P<0.05).DCFH-DA fluorescence probe assays confirmed the reactive oxygen species(ROS)-scavenging capacity of EPL-LLA(P<0.05).Conclusion EPL-LLA may treat periodontitis through multi-component,multi-target,and multi-pathway mechanisms,providing a theoretical basis for further research on its therapeutic potential.关键词
牙周炎/墨旱莲/女贞子/网络药理学/分子对接/巨噬细胞/炎症因子/氧化应激Key words
periodontitis/Eclipta prostrata L/Ligustrum lucidum Ait/network pharmacology/molecular docking/macrophage/inflammatory factors/oxidative stress分类
医药卫生引用本文复制引用
郭梦茹,张天翼,黄靖雯,黄莘越,郑义,张莉..墨旱莲—女贞子治疗牙周炎的潜在成分及作用机制[J].华西口腔医学杂志,2025,43(5):696-710,15.基金项目
Jilin Province Science and Technology Development Plan Project(YDZJ202301ZYTS013) （YDZJ202301ZYTS013）
Scientific Re-search Project of Education Department of Jilin Province(JJKH20250203KJ) 吉林省科技发展计划项目(YDZJ202301ZYTS013) （JJKH20250203KJ）
吉林省教育厅科学技术研究项目(JJKH20250203KJ) （JJKH20250203KJ）
