华西口腔医学杂志2025，Vol.43Issue(5)：728-735,8.DOI:10.7518/hxkq.2025.2025084
谷胱甘肽转移酶通过代谢重编程增强变异链球菌环境适应性的功能研究
Metabolic reprogramming by glutathione S-transferase enhances environmental adaptation of Streptococcus mu-tans
摘要
Abstract
Objective This study aims to investigate the impact of glutathione S-transferase(GST)on the environ-mental adaptability of Streptococcus mutans(S.mutans).Methods A GST knockout strain ΔgsT was constructed.Tran-scriptomic sequencing was performed to analyze the gene expression differences between the wild-type S.mutans UA159 and its GST knockout strain ΔgsT.Comprehensive functional assessments,including acid tolerance assays,hy-drogen peroxide challenge assays,nutrient limitation growth assays,and fluorescence in situ hybridization,were conduct-ed to evaluate the acid tolerance,antioxidant stress resistance,growth kinetics,and interspecies competitive ability of ΔgsT within plaque biofilms.Results Compared with the wild-type S.mutans,198 genes in ΔgsT were significantly differentially expressed and enriched in pathways related to metabolism,stress response,and energy homeostasis.The survival rate of ΔgsT in acid tolerance assays was markedly reduced(P<0.01).After 15 min of hydrogen peroxide challenge,the survival rate of ΔgsT decreased to 38.12%(wild type,71.75%).Under nutrient-limiting condi-tions,ΔgsT exhibited a significantly lower final OD600 value than the wild-type strain(P<0.05).In the biofilm competi-tion assays,the proportion of S.mutans ΔgsT in the mixed biofilm(8.50%)was significantly lower than that of the wild type(16.89%)(P<0.05).Conclusion GST enhances the acid resistance,oxidative stress tolerance,and nutrient adapta-tion of S.mutans by regulating metabolism-related and stress response-related genes.关键词
谷胱甘肽转移酶/变异链球菌/耐酸性/氧化应激/种间竞争/基因表达调控/代谢重编程Key words
glutathione S-transferase/Streptococcus mutans/acid tolerance/oxidative stress/interspecies competition/gene expression regulation/metabolic reprogramming分类
医药卫生引用本文复制引用
郑皓月,彭显,邹静..谷胱甘肽转移酶通过代谢重编程增强变异链球菌环境适应性的功能研究[J].华西口腔医学杂志,2025,43(5):728-735,8.基金项目
National Natural Science Foundation of China(82170947) 国家自然科学基金(82170947) （82170947）
