经济动物学报2025，Vol.29Issue(5)：373-380,8.DOI:10.13326/j.jea.2025.1813
TGF-β/Smad信号通路对吉林白鹅胚胎期背部皮肤毛囊发育的影响
Role of TGF-β/Smad Signaling Pathway in Development of Back Skin Hair Follicles of Jilin White Goose at Embryonic Stage
摘要
Abstract
TGF-β/Smad signaling pathway is a classical transmembrane signaling pathway that can affect cell proliferation,differentiation,and apoptosis.To investigate the mechanism of the TGF-β/Smad signaling pathway in the development of embryonic skin feather follicles in poultry,Jilin white geese were selected as the experimental animals.A combination of tissue staining,expression quantification,and localization analysis was employed to detect the expression of genes in the TGF-β/Smad signaling pathway during the morphological development of hair follicles.The results showed that during the early stages of skin hair follicle development in goose embryos,the downstream transcription factor Smad3,downstream of the TGF-β/Smad signaling pathway,was expressed in the entire skin tissue,including the epidermis and dermis,and Smad3 concentrated in the inner and outer root sheath of the feather follicle.Western blot results showed that the phosphorylated protein P-Smad3 was significantly more highly expressed in the later stages of embryonic development than in the earlier stages,indicating that the activity of the TGF-β/Smad signaling pathway genes was higher in the last stages of embryonic development than in the earlier stages.When the TGF-β/Smad signaling pathway activator was injected into the yolk sac of the embryo,it was found that the activation of the TGF-β/Smad signaling pathway inhibited the development of feather follicles.In conclusion,TGF-β/Smad signaling pathway can affect the development of the feather follicles of the goose.关键词
吉林白鹅/TGF-β/Smad信号通路/毛囊发育Key words
Jilin white goose/TGF-β/Smad signaling pathway/follicle development分类
农业科技引用本文复制引用
刘托娅,孙永峰,付献欧,周宇轩,林广宇,刘春亮..TGF-β/Smad信号通路对吉林白鹅胚胎期背部皮肤毛囊发育的影响[J].经济动物学报,2025,29(5):373-380,8.基金项目
吉林省科技发展计划重点研发项目(20230202064NC),吉林省人力资源和社会保障厅创新创业人才资助项目(2022ZY16),吉林省畜牧管理局畜禽遗传资源开发利用项目(2023) （20230202064NC）
