经济动物学报2025，Vol.29Issue(5)：392-399,8.DOI:10.13326/j.jea.2025.1832
牛传染性鼻气管炎病毒感染MDBK细胞的转录组差异表达分析
Transcriptome Analysis of MDBK Cells Infected with Infectious Bovine Rhinotracheitis Virus
摘要
Abstract
The infection of cattle with bovine rhinotracheitis virus can cause immunosuppression and lead to secondary infections that cause bacterial pneumonia and bovine respiratory disease syndrome,which cause serious economic losses to the global cattle industry.To investigate the molecular mechanism of IBRV infection in host cells,MDBK cells were inoculated with IBRV NM8 strain and the cellular differential transcriptome data were obtained by high-throughput sequencing,and analyzed for functional enrichment of differentially expressed genes.The results showed that compared with the control group,there were a total of 3 518 differentially expressed genes in the IBRV NM8 strain infected group,including 2 027 up-regulated genes and 1 491 down-regulated genes.By GO annotation and KEGG pathway enrichment analysis,it was found that the differential genes in the IBRV NM8 strain infected group were mainly involved in viral protein interaction with cytokine and cytokine receptor,lysosome,and p53 signaling pathway.By flow cytometry,it was found that infection of MDBK cells by IBRV NM8 strain could cause apoptosis of the cells.The results of the study provide a reference for in-depth analysis of the pathogenesis of IBRV.关键词
牛传染性鼻气管炎病毒/MDBK细胞/转录组分析Key words
infectious bovine rhinotracheitis virus/MDBK cells/transcriptome analysis分类
农业科技引用本文复制引用
马青霞,冷雪,杜锐,汪婷婷,刘宏莹,许立慧,刘莹玉,崔珍珍,宫庆龙,李健明,时坤..牛传染性鼻气管炎病毒感染MDBK细胞的转录组差异表达分析[J].经济动物学报,2025,29(5):392-399,8.基金项目
吉林省自然科学基金项目(20220101332JC),吉林省科技发展计划项目(20190301004NY) （20220101332JC）
