经济动物学报2025，Vol.29Issue(5)：400-405,6.DOI:10.13326/j.jea.2025.1859
黑龙江地区貉源大肠杆菌喹诺酮类药物耐药基因检测与分析
Detection and Analysis of Quinolone Drug-Resistant Genes in Escherichia coli from Raccoon Dogs in Heilongjiang Area
摘要
Abstract
In order to analyze the distribution of quinolone drug resistance genes in E.coli from raccoon dogs in Heilongjiang area,150 strains of E.coli were isolated from the racoon dogs in four farms in Heilongjiang Province.The quinolone drug resistance genes PMQR and QRDR were detected and analyzed by PCR.The results showed that PMQR resistance genes qnrB,qnrS,oqxA,oqxB,aac(6′)-Ib-cr were detected.The detection rates were 0.67%(1/150),24.00%(36/150),26.67%(40/150),40.00%(60/150),46.00%(69/150),respectively.No qnrA and qepA genes were detected.In addition,QRDR resistance genes GyrA,GyrB,ParC and ParE were detected,with detection rates of 11.33%(17/150),60.00%(90/150),36.67%(55/150)and 61.33%(92/150),respectively.The results showed that PMQR drug resistance gene aac(6′)-Ib-cr and QRDR drug resistance gene ParE were the main quinolone resistance genes in four raccoon farms,and there is a highly mutable site at serine 83 of GyrA.关键词
貉/大肠杆菌/喹诺酮药物/耐药基因/耐药性Key words
raccoon dog/Escherichia coli/quinolone/drug resistance gene/drug resistance分类
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栾欣媛,李涛,董文祥,王东阳,周航,薛原..黑龙江地区貉源大肠杆菌喹诺酮类药物耐药基因检测与分析[J].经济动物学报,2025,29(5):400-405,6.基金项目
东北林业大学大学生创新创业项目(202310225524) （202310225524）
