军事医学2025，Vol.49Issue(9)：647-654,8.DOI:10.7644/j.issn.1674-9960.2025.09.002
光生物调节对慢性应激所致认知功能障碍的改善作用及机制研究
Ameliorative effect and mechanism of photobiomodulation on cognitive dysfunction caused by chronic stress
董华丰 1刘冰 1陈小冰 1刘薇薇 1谢方 1赵云 1孙兆炜 1王雪 1钱令嘉1
作者信息
- 1. 军事科学院军事医学研究院,北京 100850
- 折叠
摘要
Abstract
Objective To find out whether photobiomodulation(PBM)can mitigate cognitive dysfunction caused by chronic stress by affecting levels of adenosine triphosphate(ATP)and adenosine receptors.Methods Twenty-four C57BL/6J mice were randomly divided into a control group,a stress group,and a treatment group.Chronic unpredictable mild stress was used to establish a mouse model of stress.Six weeks into modeling,the treatment group was subjected to one week of PBM interventions.Behavioral tests were conducted to observe behavioral changes in the mice.Western blotting(WB)was used to detect the expressions of A1,A2B,and A3 adenosine receptors in the hippocampus and prefrontal cortex of mice in the three groups.Twelve C57BL/6J mice were randomly divided into a control group and an intervention group.The intervention group received a week of PBM interventions and underwent behavioral testing.WB was used to detect the expression changes of A1,A2B,and A3 adenosine receptors in the hippocampus and prefrontal cortex in both groups.Immunofluorescence assay was adopted to detect the expression of c-Fos in the hippocampus of mice in the two groups.The ATP assay kit made by Beyotime Biotechnology Co.,Ltd.was used to measure changes in ATP contents in the hippocampus and prefrontal cortex tissues of mice.Cell experiments were conducted to verify the effect of PBM on intracellular ATP contents.Results Mice in the stress group covered a similar distance to the control group,but finished far fewer platform crossings.There was no significant difference between the treatment group and the control group in the number of times of platform crossings,but compared favorably with the stress group where the levels of adenosine receptors in the hippocampus and prefrontal cortex were lower,but were increased by PBM.After PBM interventions in normal mice,platform crossings were increased significantly compared to the control group.PBM also raised adenosine receptor levels and ATP contents in the hippocampus and prefrontal cortex,and increased hippocampal c-Fos expressions.In vitro,PBM elevated intracellular ATP levels.Conclusion PBM may improve chronic stress-induced cognitive dysfunction by regulating ATP levels and adenosine receptor expressions,thereby modulating neuronal responsiveness in the hippocampus.关键词
光生物调节/慢性应激/星形胶质细胞/腺苷受体Key words
photobiomodulation/chronic stress/astrocytes/adenosine receptors分类
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董华丰,刘冰,陈小冰,刘薇薇,谢方,赵云,孙兆炜,王雪,钱令嘉..光生物调节对慢性应激所致认知功能障碍的改善作用及机制研究[J].军事医学,2025,49(9):647-654,8.
