军事医学2025，Vol.49Issue(9)：674-680,7.DOI:10.7644/j.issn.1674-9960.2025.09.005
基于RPA-CRISPR/Cas12a的食品中肉毒杆菌快速检测方法建立
RPA-CRISPR/Cas12a-based rapid detection of Clostridium botulinum in food
摘要
Abstract
Objective To establish a rapid detection method for Clostridium botulinum in food.Methods A rapid detection method based on RPA-CRISPR/Cas12a was developed by integrating recombinase polymerase amplification(RPA)with the CRISPR-Cas12a system.After the reaction conditions were optimized,the method's sensitivity,specificity,and usefulness were methodically confirmed.Results and Conclusion The optimized method achieved detection within 1 hour,with a limit of detection(LOD)of 1.91 copies/μL.No cross-reactivity was observed with non-target pathogens.The RPA-CRISPR/Cas12a-based detection method developed in this study exhibits high specificity,sensitivity,and operational simplicity and may provide a feasible solution for the rapid detection of foodborne pathogens.关键词
食品/肉毒杆菌/规律成簇的间隔短回文重复技术/快速检测Key words
food/Clostridium botulinum/CRISPR-Cas12a/rapid detection分类
医药卫生引用本文复制引用
兰紫舒,周树青,陈天姣,谭蓉,金敏,杨栋..基于RPA-CRISPR/Cas12a的食品中肉毒杆菌快速检测方法建立[J].军事医学,2025,49(9):674-680,7.基金项目
国家重点研发计划重点专项(2023YFC3205800) （2023YFC3205800）
