军事医学2025，Vol.49Issue(9)：681-686,6.DOI:10.7644/j.issn.1674-9960.2025.09.006
UPLC-MS联用动态检测急性利血平注射诱导单胺递质耗竭模型小鼠血清中的NE和5-HT含量
Dynamic detection of NE and 5-HT levels in the serum of acute reserpine-induced depression-like mice by UPLC-MS coupling
骆芙瑶 1金子佳 2高春雪 3徐锐 1张有志 1李长伟 1朱帅鸣1
作者信息
- 1. 军事科学院军事医学研究院国家安全特需药品全国重点实验室,北京 100850
- 2. 北京工商大学,北京 100048
- 3. 华北理工大学,河北唐山 063210
- 折叠
摘要
Abstract
Objective To develop an ultra high performance liquid chromatography-mass spectrometry(UPLC-MS)method for quantifying serum levels of norepinephrine(NE)and 5-hydroxytryptamine(5-HT),and to monitor the dynamic changes in these neurotransmitters during the process of establishing a model of acute reserpine-induced depression-like mice.Methods By evaluating matrix effect,recovery,precision,and accuracy efficiency,an UPLC-MS method for determining the concentrations of NE and 5-HT in serum was established.Forty-eight C57 mice were randomly divided into normal control and model groups,which were intraperitoneally injected with physiological saline(10 mL/kg)and reserpine(2.5 mg/kg),respectively.At various time points after intraperitoneal injection,the degree of ptosis and decreases in body temperature of the mice were observed before orbital blood was sample for the determination of NE and 5-HT levels.Results The concentrations of NE and 5-HT showed good linearity within the range of 15.63 to 2000.00 ng/mL,with R2 values greater than 0.999.The results of methodological validation met the requirements for the analysis of biological samples,with a lower limit of quantification of 15.63 ng/mg.After intraperitoneal injection of reserpine,the model mice exhibited varying body temperature decreases and ptosis.At 1 and 2 h post-administration,the depression-like symptoms in the model group were significantly different from those of the normal control group(P＜0.01).The body temperature of mice in the model group was significantly lower than that of mice in the normal control group(P＜0.01),while the score of the eyelid ptosis was significantly higher(P＜0.001).The levels of NE and 5-HT in the serum of model mice were also significantly depleted,and were significantly different from those of the normal control group at 0.5,1 and 2 h(P＜0.05).Conclusion The study process of established a rapid and accurate method for dynamically observing the changes in NE and 5-HT levels during the process of establishing a model of acute reserpine-induced depression-like mice,which might contribute to the study of the pathogenesis of depression and the development of new antidepressant drugs.关键词
超高效液相色谱-质谱/去甲肾上腺素/5-羟色胺/利血平/抑郁Key words
ultra high performance liquid chromatography-mass spectrometry/norepinephrine/5-hydroxytryptamine/reserpine/depression分类
医药卫生引用本文复制引用
骆芙瑶,金子佳,高春雪,徐锐,张有志,李长伟,朱帅鸣..UPLC-MS联用动态检测急性利血平注射诱导单胺递质耗竭模型小鼠血清中的NE和5-HT含量[J].军事医学,2025,49(9):681-686,6.
