菌物学报2025，Vol.44Issue(10)：31-45,15.DOI:10.13346/j.mycosystema.250092
种间原生质体融合选育高活性成分的灵芝新菌株
Breeding of novel strains of Ganoderma spp.with high active ingredients by means of interspecific protoplast fusion
摘要
Abstract
Protoplast fusion between high ergothioneine(EGT)-producing Ganoderma strains,Ganoderma resinaceum FQ23 and Ganoderma sessile FQ16 was performed,and 10 fusion strains(RS1,RS6,RS7,RS8,RS10,RS11,RS12,RS13,RS14,and RS16)were obtained.Substrate cultivation and solid fermentation of both fusion strains and parental stains were conducted.The key bioactive compounds including ergothioneine,triterpenoids and polysaccharides were quantitatively analyzed and systematically compared.Incorporating characteristic radar chart analysis,correlation matrix assessment,amino acid composition profiling,and nutritional value evaluation were carried out to obtain the strains suitable for substrate cultivation and solid fermentation.The results showed that fusion strains RS6 and RS8 were the most suitable for substrate cultivation.It was found that RS6 had the highest polyphenol content in the fruiting body,being 28.97％and 20.45％higher than that in the two parents FQ23 and FQ16,respectively;the EGT content is also higher,which is 3.05％and 4.02％higher than that in the two parents,respectively.RS16 was the most suitable for solid fermentation because the content of EGT was the highest in solid fermentation substrate,being 52.40％and 38.84％higher than that of the two parents FQ23 and FQ16,respectively;besides,the polysaccharide content has increased by 23.80％and 28.07％,respectively.The experimental findings demonstrated that our protoplast fusion breeding was successful and practicable for modulating the bioactive compounds in medicinal fungi.关键词
树脂灵芝/无柄灵芝/子实体栽培/固体发酵/原生质体融合育种/活性成分Key words
Ganoderma resinaceum/Ganoderma sessile/substrate cultivation/solid fermentation/protoplast fusion breeding/active component引用本文复制引用
周凤龙,梁雅婷,严香丽,叶志伟,郭丽琼,林俊芳..种间原生质体融合选育高活性成分的灵芝新菌株[J].菌物学报,2025,44(10):31-45,15.基金项目
国家自然科学基金(32072646,32272785) This work was supported by the National Natural Science Foundation of China(32072646,32272785). （32072646,32272785）
