菌物学报2025，Vol.44Issue(10)：63-74,12.DOI:10.13346/j.mycosystema.250102
灵芝C2H2转录因子GlZF1的功能分析
Functional analyses of C2H2 transcription factor GlZF1 in Ganoderma lingzhi
摘要
Abstract
Ganoderic acid,as one of the key active ingredients in Ganoderma lingzhi,holds significant development value in the fields of medicine and health care.Zinc finger proteins(ZFPs)constitute one of the largest transcription factor families in eukaryotic genomes.In which C2H2-type zinc finger proteins play crucial roles in regulating plant growth and development,and response to environmental stresses,and have proven to be effective in regulating the synthesis of secondary metabolites.In this study,the C2H2 transcription factor GlZF1 gene was successfully obtained from G.lingzhi through homologous sequence alignment,and its function was analyzed using RNA interference(RNAi)technology.The results demonstrated that the content of ganoderic acid in the GlZF1 RNAi transformants increased by an average of 26％compared with that in the wild-type strains.The expression levels of key enzyme genes in the ganoderic acid biosynthetic pathway,including 3-hydroxy-3-methylglutaryl-CoA synthase(hmgs),3-hydroxy-3-methylglutaryl coenzyme-A reductase(hmgr),farnesyl pyrophosphate synthase(fps),squalene synthase(sqs),and oxidosqualene cyclase(osc),were significantly upregulated in the RNAi transformants.In addition,the enzyme activity of peroxidase(POD)and the level of reactive oxygen species(ROS)increased significantly in the transformants.However,the enzyme activity of superoxide dismutase(SOD)and the content of proline decreased significantly in the transformants.The expression levels of antioxidant-related genes,such as catalase(CAT1,CAT2),glutathione peroxidase(GPx),and NADPH peroxidase(NoxR)gene generating reactive oxygen species increased significantly in the transformants.The results indicated that C2H2 transcription factor GlZF1 of G.lingzhi not only regulated the biosynthesis of ganoderic acids,but also had a significant effect on the antioxidant capacity in G.lingzhi.The results provide a preliminary basis for further understanding of the regulatory mechanism of the secondary metabolic pathway and the stress resistance mechanism against adverse environments of G.lingzhi.关键词
灵芝/C2H2 型锌指蛋白/灵芝酸/次生代谢/抗胁迫能力/活性氧Key words
Ganoderma lingzhi/C2H2 type zinc finger protein/ganoderic acid/secondary metabolism/stress resistance/reactive oxygen species引用本文复制引用
孙京伟,李华君,王欣杰,张佩锦,梁力丹,史青云,常思源,孟丽..灵芝C2H2转录因子GlZF1的功能分析[J].菌物学报,2025,44(10):63-74,12.基金项目
山东省自然科学基金(ZR2024MC167) This work was supported by the Shandong Provincial Natural Science Foundation(ZR2024MC167). （ZR2024MC167）
