菌物学报2025，Vol.44Issue(10)：75-89,15.DOI:10.13346/j.mycosystema.250049
皱环球盖菇酪氨酸酶基因家族鉴定及表达分析
Identification and expression analyses of the tyrosinase gene family in Stropharia rugosoannulata
摘要
Abstract
The tyrosinase(TYR)gene family plays a crucial role in color formation and various physiological processes in fungi.In this study,a genome-wide identification and bioinformatics analysis of potential TYR genes in Stropharia rugosoannulata were conducted.Additionally,the relative expression levels of TYR family members at different developmental stages and in various parts of the fruiting bodies with different colors were investigated.In total,eight SrTYRs members were identified in the S.rugosoannulata genome.They were located on five chromosomes with a clustered distribution pattern.All eight genes contain numerous cis-element related to light-response and hormone-response in the upstream promoter regions.The Sr TYRs exhibited differences in conserved motifs,signal peptides,subcellular localization,and physicochemical properties,yet shared similar three-dimensional structures.With the exception of Sr TYR7,all members possess two highly conserved copper-binding sites.Phylogenetic tree analysis indicated that the Sr TYRs could be divided into two classes.The expression levels of SrTYRs were low during the mycelial and primordial stages.However,SrTYR2,SrTYR5,SrTYR6,and SrTYR7 were highly expressed after the formation of fruiting bodies.Notably,SrTYR5,SrTYR6,and SrTYR7 showed significantly higher relative expression levels in the pileipellis compared to the cap,gills,and stipe,consistent with the observed sites of pigment accumulation,suggesting their potential function in color formation.关键词
皱环球盖菇/TYR基因家族/生物信息学/相对表达量Key words
Stropharia rugosoannulata/TYR gene family/bioinformatics/relative expression level引用本文复制引用
谢振飞,张国庆,张薇薇,赵顺银,李念祖,李佳书..皱环球盖菇酪氨酸酶基因家族鉴定及表达分析[J].菌物学报,2025,44(10):75-89,15.基金项目
北京自然科学基金(6232003,6244037) （6232003,6244037）
现代农业产业体系北京市食用菌创新团队(BAIC03) This work was supported by the Beijing Natural Science Foundation(6232003,6244037)and the Beijing Innovation Consortium of Agriculture Research System(BAIC03). （BAIC03）
