菌物学报2025，Vol.44Issue(10)：90-101,12.DOI:10.13346/j.mycosystema.250066
油茶果生刺盘孢组蛋白甲基转移酶CfSet9生物学功能
Functional characterization of histone methyltransferase Set9 in Colletotrichum fructicola,the causal agent of Camellia oleifera anthracnose
摘要
Abstract
Anthracnose is the main disease in Camellia oleifera planting area,caused by Colletotrichum fructicola.As an important epigenetic modification,histone methylation plays an important role in the regulation of a variety of biological processes,which is currently unclear in C.fructicola.This study investigates the biological functions of the histone methyltransferase CfSet9 in C.fructicola and provides critical insights into the molecular mechanisms by which CfSet9 regulates pathogenicity.The ΔCfset9 mutant was generated via homologous recombination and PEG-mediated protoplast transformation,followed by the successful construction of the complemented strains ΔCfset9/CfSET9.The research found that CfSet9 was responsible for regulating the trimethylation of histone H4 lysine20(H4K20me3).Biological phenotype determination indicates that the growth rate of the CfSET9 gene knockout mutant does not exhibit significant differences in comparison with the wild-type and complement strains,but its conidiation and appressorial formation rate were significantly reduced.The ΔCfset9 mutant was more sensitive to oxidation,osmotic pressure,and cell wall stress and more tolerant to DNA damage environment(MMS).The ΔCfset9 mutant exhibited significant reduction in pathogenicity.Taken together,our results revealed the critical roles of histone methyltransferase CfSet9 in conidiation,appressorial formation,appressorial turgor,external stress responses,and pathogenicity of C.fructicola.关键词
油茶/果生刺盘孢/组蛋白甲基转移酶CfSet9/致病力Key words
Camellia oleifera/Colletotrichum fructicola/histone methyltransferase CfSet9/pathogenicity引用本文复制引用
雷美,刘瑞峰,李河..油茶果生刺盘孢组蛋白甲基转移酶CfSet9生物学功能[J].菌物学报,2025,44(10):90-101,12.基金项目
国家重点研发计划(2023YFD1401301) This work was supported by the National Key Research and Development Program of China(2023YFD1401301). （2023YFD1401301）
