菌物学报2025，Vol.44Issue(10)：102-113,12.DOI:10.13346/j.mycosystema.250059
蚓粪生木霉TC467拮抗植物病原真菌、促生长及杀菌剂耐受性初探
Trichoderma vermifimicola strain TC467 possessing phytopathogen antagonism,plant growth promotion and fungicide resistance
摘要
Abstract
Trichoderma strains possessing biological control functions have been used in agriculture against phytopathogens.Currently,only very few species of the genus were applied to or involved in plant disease control.Discovery of additional useful resources is desperately needed.In this study,biocontrol effect of Trichoderma vermifimicola strain TC467 was evaluated by dual confrontation culture,cellophane and two-compartment culture,pot experiments,and resistance to chemical fungicides.The results demonstrated that TC467 produced substances essential to phytopathogen control(including siderophore,xylanase and chitinase)and plant growth promoters(producing indole-3-acetic acid and gibberellin).The strain displayed a high inhibition rate against Botrytis cinerea reaching 85.26％;and its non-volatile and volatile secondary metabolites showed the inhibition rates to Sclerotinia sclerotiorum and B.cinerea as high as 84.67％and 47.62％,respectively.In pot experiments,comparing with untreated plants TC467 significantly enhanced the height and fresh weight of lettuce(Lactuca sativa var.ramosa)by 46.69％and 15.33％,respectively.Its fermentation broth effectively minimized the lettuce disease caused by B.cinerea with inhibition rate of 87.76％.In addition,the strain showed higher tolerance to hymexazol water-dispersible granule than that to other tested fungicides;at the concentration of 0.42 mg/L the growth rate of TC467 can even approach 98.19％.T.vermifimicola strain TC467 has the potential for practical application in biocontrol especially plant diseases caused by B.cinerea,which extends our knowledge of nature beneficial resources.关键词
生物防治/木霉/灰葡萄孢/生长抑制率/植物病害Key words
biological control/Trichoderma/Botrytis cinerea/growth inhibition rate/plant diseases引用本文复制引用
王恒煦,曾昭清,庄文颖..蚓粪生木霉TC467拮抗植物病原真菌、促生长及杀菌剂耐受性初探[J].菌物学报,2025,44(10):102-113,12.基金项目
国家重点研发计划(2022YFC2303000) This work was supported by the National Key Research and Development Program of China(2022YFC2303000). （2022YFC2303000）
