菌物学报2025，Vol.44Issue(10)：114-125,12.DOI:10.13346/j.mycosystema.250006
新渡户树蜂两种共生真菌的形态及降解木质纤维素能力
Morphology and lignocellulose degradation ability of two symbiotic fungi associated with Sirex nitobei
摘要
Abstract
The symbiotic fungi secrete enzymes that degrade lignocellulose to help the larvae of the Sirex insect to gain food.In this study,the morphology,growth rate,and lignocellulose utilization capacity of the two symbiotic fungi Amylostereum areolatum and A.chailletii associated with Sirex nitobei,the major boring pest of coniferous trees,were studied,and the influence of the symbiotic fungi on the growth and development of the insect larvae was also investigated.The results showed that the growth rate of A.areolatum was slower than that of A.chailletii;the hyphae of the two fungi grown on the medium were all white,but A.areolatum turned the matrix yellow,and A.chailletii turned the matrix white.There was clamp connection in the two fungi;Hyphae of A.areolatum fragmented after a period of culture.A.chailletii degraded lignocellulose better than A.areolatum.Lignin peroxidase(LiP)of A.areolatum maintained high level on day 5 and 10,reaching a maximum level on day 10;the manganese peroxidase(MnP)reaches its maximum peak on day 5;the laccase(Lac)showed a trend of increase at the initial stage and decrease at later stage,and the enzyme activity was stable in the first 10 days,and reached its maximum on the 15th day.The LiP enzyme of A.chailletii reached its maximum activity on the 5th day,and then showed a downward trend reaching the lowest level on the 25th day;MnP enzyme had the highest activity on day 10 and then decreased.The enzyme activity of Lac peaked on day 20,and showed relatively stable in the early stage.The carboxymethylcellulase(CMCase)activity of the two fungi reached peak on day 5.Filter paper enzymes(FPase)activity trended to increase at the initial stage and decrease at the later stage,reaching its maximum on day 10 for A.areolatum,and on day 15 for A.chailletii.These results indicate that the two symbiotic fungi of S.nitobei have the ability to degrade lignocellulose,and the results probably provide a new idea for the control of S.nitobei by using symbiotic fungi.关键词
新渡户树蜂/共生真菌/木质纤维素酶/酶活性测定Key words
Sirex nitobei/symbiotic fungi/lignocellulolytic enzyme/enzyme activity assay引用本文复制引用
彭云娟,柴建峰,汤佳怡,耿菲,魏文婧,李载徽,王立祥..新渡户树蜂两种共生真菌的形态及降解木质纤维素能力[J].菌物学报,2025,44(10):114-125,12.基金项目
国家自然科学基金(32160379) （32160379）
甘肃农业大学伏羲人才专项(Gaufx-04Y08) This work was supported by the National Natural Science Foundation of China(32160379)and the Fuxi Talent Project of Gansu Agricultural University(Gaufx-04Y08). （Gaufx-04Y08）
