山东农业大学学报（自然科学版）2025，Vol.56Issue(5)：731-737,7.DOI:10.3969/j.issn.1000-2324.2025.05.001
双修饰三螺旋DNA荧光分子开关高灵敏检测玉米Bt转基因
Dual-Modified Triple Helix DNA Fluorescence Molecular Switch for Highly Sensitive Detection of Bt Transgene in Maize
摘要
Abstract
The Bacillus thuringiensis(Bt)gene,an important exogenous gene in insect-resistant transgenic crops,plays a crucial role in crop pest control.The cultivation and promotion of Bt transgenic crops have greatly reduced the use of synthetic insecticides and brought huge benefits to farmers.However,the safety of Bt transgenic crops has gradually attracted people's attention.Therefore,it is of great significance to develop a simple and sensitive method for detection of Bt transgene in crops.This study constructs a dual-modified triple helix DNA fluorescent molecular switch(DTHFMS)and systematically investigates the influencing factors and variation rules of the stability of DTHFMS.The results show that DTHFMS with alternating triplets of T-AoT and C-GoC demonstrates proper stability,making it suitable for highly sensitive detection of Bt transgenes in maize seeds.This study achieves excellent detection results by optimizing key detection parameters.The limit of detection is 0.05 pM,with a linear range of(0.1-6.0×104)pM.关键词
苏云金芽孢杆菌/三螺旋DNA/分子开关/高灵敏检测Key words
Bacillus thuringiensis/triple helix DNA/molecular switch/highly sensitive detection分类
化学化工引用本文复制引用
张非凡,褚秀玲,张川,陆羽新,朱德颂..双修饰三螺旋DNA荧光分子开关高灵敏检测玉米Bt转基因[J].山东农业大学学报（自然科学版）,2025,56(5):731-737,7.基金项目
中国博士后面上资助项目(2018M632697) （2018M632697）
泰安市科技创新发展项目(2021NS362) （2021NS362）
