山东农业大学学报（自然科学版）2025，Vol.56Issue(5)：774-780,7.DOI:10.3969/j.issn.1000-2324.2025.05.005
碳点对四环素类抗生素的荧光检测
Fluorescence Detection of Tetracycline Antibiotics by Carbon Dots
史泽琨 1姜良妹 2郝晨昱 2李丹 3王明林 1侯芹2
作者信息
- 1. 山东农业大学食品科学与工程学院,山东 泰安 271018
- 2. 山东农业大学化学与材料科学学院,山东 泰安 271018
- 3. 山东医药技师学院,山东 泰安 271016
- 折叠
摘要
Abstract
Tetracycline antibiotics(TCs)are a commonly used class of broad-spectrum antibiotics.Due to their broad-spectrum antibacterial properties,they are widely applied in multiple industries such as medical treatment and animal husbandry.However,excessive use can lead to the residue of TCs,posing a serious threat to the ecological environment and human health.Therefore,it is of great significance to develop a simple,efficient,rapid,and sensitive method to detect TCs residues for monitoring the environment and food safety.Fluorescence sensing methods have the advantages of obvious phenomena,high sensitivity and convenient operation.This paper synthesizes carbon dots by one-step hydrothermal method with citric acid and ethylenediamine as precursors.It characterizes the carbon dots by infrared spectroscopy,ultraviolet spectroscopy,powder X-ray diffraction,and fluorescence spectroscopy.Their optimal excitation wavelength is determined as 350 nm,with the maximum emission wavelength of 450 nm,exhibiting blue fluorescence.Moreover,this paper employs carbon dots for fluorescence detection of tetracycline,chlortetracycline,and oxytetracycline.The blue fluorescence gradually quenches when carbon dots interact with TCs.Within the range of 0-250 µM,the detection limits of tetracycline,chlortetracycline,and oxytetracycline are 720 nM,747 nM,and 765 nM,respectively.This paper also investigates the optimal detection conditions and the influence of possible interfering ions on TCs detection.The results show that the carbon dots exhibit significant selectivity for TCs.关键词
四环素/碳点/荧光检测/高灵敏/高选择Key words
Tetracycline antibiotics/carbon dots/fluorescence detection/high sensitivity/high selectivity分类
化学化工引用本文复制引用
史泽琨,姜良妹,郝晨昱,李丹,王明林,侯芹..碳点对四环素类抗生素的荧光检测[J].山东农业大学学报（自然科学版）,2025,56(5):774-780,7.
