山东农业大学学报（自然科学版）2025，Vol.56Issue(5)：801-809,9.DOI:10.3969/j.issn.1000-2324.2025.05.008
两株生防菌对西瓜枯萎病的防效及其促生作用
Control Efficacy and Growth Promotion of Two Biocontrol Bacteria Against Fusarium Wilt of Watermelon
摘要
Abstract
To verify the pathogenic characteristics of Fusarium oxysporum BMD-1 and explore the biocontrol and growth promotion effects of Bacillus amylolifacium B6 and Pseudomonas WF19 on watermelon plants infected with BMD-1,this study first tests the pathogenic ability of BMD-1 pathogen by inoculation method,and measures the nitrogen metabolism,antioxidant enzyme activities,malondialdehyde and lignin contents of watermelon plants by pot experiment.The results show that 5-7 days after BMD-1 infection,the seedling roots turn brown and their growth and development are inhibited,indicating that BMD-1 is pathogenic to watermelon plants.The dry weight and fresh weight of watermelon plants above and below ground significantly increase by the addition of strain B6,WF19 and their composite strain BW.The fresh weight above and below ground increase by 35.79％and 51.85％respectively in B6 treatment,and by 28.41％and 123.46％respectively in WF19 treatment.Meanwhile,the contents of MDA and NH4+-N in the plants decrease,and the activities of nitrogen metabolism enzymes NR,GDH,GS and antioxidant enzymes POD,PPO,PAL improve.Among these,B6 exhibits the best overall effect.In summary,strains B6 and WF19 can promote the growth and development of watermelon plants by regulating nitrogen metabolism,antioxidant enzyme activities and lipid peroxidation to improve watermelon resistance to fusarium wilt.关键词
枯萎病/生防菌/生防效果Key words
Fusarium wilt/biocontrol bacterium/biocontrol effect分类
农业科技引用本文复制引用
刘倩如,沈婷婷,张琇,齐玉玺,田兴国,禹凤霞,杨国平..两株生防菌对西瓜枯萎病的防效及其促生作用[J].山东农业大学学报（自然科学版）,2025,56(5):801-809,9.基金项目
宁夏自然科学基金重点项目(2022AAC02048) （2022AAC02048）
国家自然科学基金项目(32060424) （32060424）
宁夏重点研发计划(2023BCF01014) （2023BCF01014）
宁夏回族自治区领军人才计划(2022GKLRLX06) （2022GKLRLX06）
