山东农业大学学报（自然科学版）2025，Vol.56Issue(5)：810-822,13.DOI:10.3969/j.issn.1000-2324.2025.05.009
5个白蜡无性系耐盐性及对NaCl胁迫的生理响应
Salt Tolerance and Physiological Response to NaCl Stress in Five Fraxinus chinensis Clones
摘要
Abstract
To study the differences in salt tolerance among different ash(Fraxinus chinensis)clones in saline and alkaline land,this study uses grafted seedlings of five clones(European Ash-Compact,European Ash-Open,12-62,12-76,and Yuanla 2)as test materials.This study simulates salt stress environments with NaCl solutions at different concentrations[1‰(CK),3‰,5‰ and 7‰],determines growth indicators,physiological and biochemical indicators and evaluates their salt tolerance.The results show that with the increasing NaCl concentration,the relative growth rate of all five ash clones decreases gradually,while biomass increases initially but decreases subsequently.The osmotic regulating substances like soluble sugar,soluble protein and free proline gradually increase.The chlorophyll content first increases and then decreases,reaching the maximum at 3‰ NaCl.The activity of superoxide dismutase(SOD)increases gradually,while the activity of peroxidase(POD)first increases and then decreases.The Na+/K+ratio increases extremely significantly.With the extension of stress time,the contents of osmotic regulating substances—soluble sugars,soluble proteins and free proline—increase significantly(P<0.05).The activities of SOD and POD enzymes first increase and then decrease,while the chlorophyll content decreases extremely significantly.Compared to the other four ash clones,clone 12-76 exhibits the highest relative seedling height growth rate,relative ground diameter increase rate,total biomass,and activities of SOD and POD enzymes,and the lowest malondialdehyde(MDA)content.A comprehensive evaluation of the growth,physiological,and biochemical indicators of different F.chinensis clones using the fuzzy membership function method shows that the salt tolerance ranking is:12-76>Yuanla 2>European Ash-Compact>European Ash-Open>Clone 12-62.关键词
白蜡/无性系/生理指标/耐盐性/综合评价Key words
Fraxinus chinensis/clones/physiological indicators/salt tolerance/comprehensive evaluation分类
农业科技引用本文复制引用
乔佳宝,梁云,秦肖辰,曹帮华,赵洪冰,侯雨含,高梦琪,毛培利..5个白蜡无性系耐盐性及对NaCl胁迫的生理响应[J].山东农业大学学报（自然科学版）,2025,56(5):810-822,13.基金项目
山东省重大科技创新工程(2017CXGC0316) （2017CXGC0316）
滨海盐碱地扩种大豆技术集成示范(152403014) （152403014）
