世界中医药2025，Vol.20Issue(13)：2274-2280,7.DOI:10.3969/j.issn.1673-7202.2025.13.007
二陈汤对小鼠结直肠癌皮下移植瘤模型的作用机制研究
Mechanisms of Erchen Decoction on Subcutaneous Transplanted Tumor Model of Colorectal Cancer in Mice
摘要
Abstract
Objective:To investigate the effects and underlying mechanisms of Erchen Decoction on subcutaneous MC38 colorectal cancer xenografts.Methods:Twenty-two C57BL/6 mice were randomly divided into a control group and an Erchen Decoction group.All mice were inoculated with MC38 colorectal cancer cells.The Erchen Decoction group received oral administration of Erchen Decoction for three weeks.Body weight,tumor volume,and tumor weight were recorded.Hematoxylin and eosin(HE)stai-ning was used to observe histopathological changes.Immunohistochemistry(IHC)was performed to detect the expression of F4/80 antigen(F4/80),inducible nitric oxide synthase(iNOS),cluster of differentiation 206 and 3(CD206,CD3),Ki-67 antigen(Ki67),CD4,CD8,and forkhead box protein P3(FoxP3)in tumor tissues.Flow cytometry(FCM)was used to assess the expression of F4/80,major histocompatibility complex class Ⅱ(MHC-Ⅱ),and CD206.Quantitative real-time PCR(qPCR)and Western blot(WB)were conducted to detect the mRNA and protein expression of tumor necrosis factor-alpha(TNF-α)and arginase-1(Arg1).Re-sults:Compared with the control group,mice in the Erchen Decoction group showed significantly reduced body weight,tumor vol-ume,and tumor mass(P＜0.05).Protein expression of F4/80,CD206,Ki67,and FoxP3 was decreased,while iNOS expression was increased(P＜0.05).The proportion of F4/80-positive cells and the mRNA and protein expression levels of Arg1 in tumor tissues were also reduced(P＜0.05).There were no statistically significant differences between the two groups in the expression of CD3,CD4,CD8,MHC-Ⅱ,CD206,or TNF-α(P＞0.05).Conclusion:Erchen Decoction inhibits the growth of subcutaneous MC38 tumors in mice,possibly by reducing the recruitment and M2 polarization of tumor-associated macrophages.关键词
结直肠癌/肿瘤相关巨噬细胞/二陈汤/皮下移植瘤/M1型肿瘤相关巨噬细胞/M2型肿瘤相关巨噬细胞/巨噬细胞募集/巨噬细胞极化Key words
Colorectal cancer/Tumor-associated macrophages/Erchen Decoction/Subcutaneous transplant tumor/M1 tumor-as-sociated macrophages/M2 tumor-associated macrophages/Macrophage recruitment/Macrophage polarization分类
医药卫生引用本文复制引用
招金娣,杨媛,魏丞,周捷,廖凌虹..二陈汤对小鼠结直肠癌皮下移植瘤模型的作用机制研究[J].世界中医药,2025,20(13):2274-2280,7.基金项目
国家重点研发计划"中医药现代化"重点专项(2023YFC3503000) （2023YFC3503000）
