世界中医药2025，Vol.20Issue(13)：2281-2288,8.DOI:10.3969/j.issn.1673-7202.2025.13.008
清心宁神汤调控磷脂酰肌醇3-激酶信号通路治疗不寐的作用机制
Mechanisms of Qingxin Ningshen Decoction in Treating Insomnia by Regulating Phosphatidylinositol 3-kinase Signaling Pathway
摘要
Abstract
Objective:To explore the potential mechanisms of Qingxin Ningshen Decoction in treating insomnia caused by heart failure in housing the spirit in rats.Methods:A model of insomnia caused by heart failure in housing the spirit was established in SD rats using caffeine combined with 4-chloro-DL-phenylalanine(PCPA).Rats were randomly divided into normal group,model group,zolpidem group,low-dose Qingxin Ningshen Decoction group,high-dose Qingxin Ningshen Decoction group,and pathway in-hibitor group,six rats in each group.The synergistic hypnotic effect of the drugs was assessed by the pentobarbital sodium sleep po-tentiation test.Protein expression levels of phosphoinositide 3-kinase(PI3K),protein kinase B(AKT),forkhead box O(FOXO),and their corresponding phosphorylated proteins in rat brain tissue were detected by Western blot.The mRNA expression levels of PI3K,AKT,and FOXO were measured by real-time quantitative PCR(qPCR).Immunohistochemistry(IHC)and immunofluores-cence(IF)assays were used to evaluate the expression and phosphorylation levels of PI3K,AKT,and FOXO in brain tissue.Re-sults:Compared with the model group,the zolpidem group,low-and high-dose Qingxin Ningshen Decoction groups,and pathway in-hibitor group showed significantly shortened sleep latency(P＜0.01)and prolonged sleep duration(P＜0.01).Expression levels of PI3K,AKT,FOXO,and their phosphorylated proteins and mRNA in brain tissue were decreased(P＜0.05 or P＜0.01).IHC and IF results demonstrated reduced expression levels and fluorescence area of PI3 K,AKT,FOXO,and corresponding phosphorylated proteins(P＜0.05 or P＜0.01).Conclusion:Qingxin Ningshen Decoction can improve sleep in rats by inhibiting the expression of factors in the PI3K/AKT/FOXO signaling pathway,with the high-dose group showing the best therapeutic effect.关键词
中医不寐五神分型诊断法/心不藏神不寐/清心宁神汤/磷脂酰肌醇3-激酶/蛋白激酶B/叉头框蛋白O/神经炎症反应/机制研究分类
医药卫生引用本文复制引用
吴金鸿,张星平,梁政亭,梁瑞宁,陈旭,贾宏林,闫德祺,胡燕玲,崔文军..清心宁神汤调控磷脂酰肌醇3-激酶信号通路治疗不寐的作用机制[J].世界中医药,2025,20(13):2281-2288,8.基金项目
国家自然科学基金项目(82160873,81960837,81560762,81260526) （82160873,81960837,81560762,81260526）
国家中医药管理局"十四五"高水平中医药重点学科项目(国中医药人教函[2023]85号) （国中医药人教函[2023]85号）
全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
新疆维吾尔自治区自然科学基金项目(2022D01D48,2020DO1C163) （2022D01D48,2020DO1C163）
