世界中医药2025，Vol.20Issue(14)：2432-2440,9.DOI:10.3969/j.issn.1673-7202.2025.14.005
当归饮子治疗寻常型银屑病的药理实验与分子生物学机制
Pharmacological Experiments and Molecular Biological Mechanisms of Danggui Yinzi in the Treatment of Psoriasis Vulgaris
摘要
Abstract
Objective:To investigate the mechanism of Danggui Yinzi in the treatment of psoriasis vulgaris(PV)based on network pharmacology,molecular docking,and animal experimental validation.Methods:The Traditional Chinese Medicine Systems Phar-macology Database and Analysis Platform(TCMSP)and the Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine 2.0(BATMAN 2.0)were used to obtain the active components and targets of Danggui Yinzi.The intersection between the targets of its active components and PV-related targets was identified.The STRING database was employed for network interaction analysis of the common targets,and a protein-protein interaction(PPI)network was constructed.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses were performed using the Metascape platform,and a"drug-active component-target"interaction network was established.Molecular docking technology and pharmacological experi-ments were used to validate the effective active components and targets.Results:A total of 163 potential target proteins of Danggui Yinzi were identified from the databases,with kaempferol and quercetin as key active components.A total of 260 common targets of Danggui Yinzi for the treatment of PV were screened,among which the potential core targets included phosphatidylinositol-3-kinase(PI3K),protein kinase B(AKT),interleukin-1β(IL-1β),interleukin-6(IL-6),and interleukin-17a(IL-17a).Molecular docking showed stable binding affinities between kaempferol,quercetin,and the predicted targets PIK3C2G and AKT1.Animal experiments demonstrated that Danggui Yinzi reduced the Psoriasis Area and Severity Index(PASI)score in PV mice,decreased serum levels of IL-1β,IL-6,and IL-17a,and downregulated the expression of PI3K,AKT,and mammalian target of rapamycin(mTOR)in skin tis-sues.Conclusion:The therapeutic mechanism of Danggui Yinzi in PV may be related to the regulation of the PI3K/AKT/mTOR signaling pathway and inflammatory mediators such as IL-1β,IL-6,and IL-17a.关键词
当归饮子/寻常型/银屑病/网络药理学/分子对接/作用机制/药理/实验验证Key words
Danggui Yinzi/Vulgaris/Psoriasis/Network pharmacology/Molecular docking/Mechanism of action/Pharmacolo-gy/Experimental verification分类
医药卫生引用本文复制引用
王栩芮,李明玥,庞尧斌,陈安婧,刘娥,赵谊佳,郭静..当归饮子治疗寻常型银屑病的药理实验与分子生物学机制[J].世界中医药,2025,20(14):2432-2440,9.基金项目
国家自然科学基金项目(82505598) （82505598）
四川省科技计划资助项目(2024NSFSC1852) （2024NSFSC1852）
成都市医学科研课题(2024287) （2024287）
国家中医药管理局"青年岐黄学者"项目(2022-256) （2022-256）
四川省人民医院基金项目(2023QN12) （2023QN12）
四川省中医药管理局科学技术研究专项(22CP1423) （22CP1423）
四川省中医药管理局项目(2021MS307) （2021MS307）
成都中医药大学"杏林学者"学科人才科研提升计划项目(QJJJ2021001 （）
成都中医药大学医院"百人计划"项目(21-L03和22-B09) （21-L03和22-B09）
成都中医药大学"基础增厚"行动计划项目(2023-42) （2023-42）
