世界中医药2025，Vol.20Issue(14)：2458-2467,10.DOI:10.3969/j.issn.1673-7202.2025.14.008
清热化痰理气方及其拆方改善细颗粒物肺损伤的机制研究
Mechanism of Qingre Huatan Liqi Formula and Its Disassembled Prescriptions in Ameliorating Fine Particulate Matter-induced Pulmonary Injury
摘要
Abstract
Objective:Based on neutrophil extracellular traps(NETs),this study aimed to investigate the mechanisms by which the Qingre Huatan Liqi Formula and its disassembled formulas(Qingre Jiedu Formula and Liqi Huatan Formula)ameliorate fine partic-ulate matter(PM2.5)-induced lung injury.Methods:A lung injury rat model was established via intratracheal instillation of PM2.5 suspension.Rats were divided into six groups:control,model,Qingre Jiedu,Liqi Huatan,Qingre Huatan Liqi,and erythromycin pos-itive control.Hematoxylin-eosin(HE)staining was used to observe lung tissue pathology.ELISA and quantitative real-time PCR(qPCR)measured interleukin-1β(IL-1β)protein and mRNA expression.ELISA also detected NET components[myeloperoxidase-DNA complexes(MPO-DNA),neutrophil elastase(NE),citrullinated histone H3(CitH3)]and NET regulatory enzymes[reactive oxygen species(ROS),NADPH oxidase,peptidylarginine deiminase 4(PAD4)].Double-stranded DNA(dsDNA)content was quantified using Picogreen fluorescence assay,and Western blot analyzed NAD(P)H dehydrogenase[quinone]1(NQO1)and MAPK pathway-related proteins.Results:All medication groups showed improved lung injury,with reductions in NET components,NET regulatory enzymes,and airway inflammatory markers.The Qingre Huatan Liqi Formula exhibited superior efficacy compared to its disassembled formulas.Specifically,the Qingre Huatan Liqi group showed greater reduction in ERK signaling activity compared to the Qingre Jiedu group and a more pronounced decrease in P38 signaling activity compared to the Liqi Huatan group(P＜0.05).Conclusion:Qingre Huatan Liqi Formula and its disassembled formulas alleviated PM2.5-induced lung injury by inhibiting NET formation through downregulation of the NADPH/ROS/histone citrullination axis and ERK/P38 MAPK signaling pathways,with the integrated formula demonstrating the optimal therapeutic effect.关键词
清热化痰理气方/清热解毒方/理气化痰方/细颗粒物/肺损伤/中性粒细胞胞外诱捕网/MAPK信号通路/瓜氨酸化组蛋白3Key words
Qingre Huatan Liqi Formula/Qingre Jiedu Formula/Liqi Huatan Formula/Fine particulate matter/Lung injury/Neutrophil extracellular traps/MAPK signaling pathway/Citrullinated histone H3分类
医药卫生引用本文复制引用
刘亚倩,冯淬灵,杨杰,王洪涛,王贵澍,郑佳昆,张永生..清热化痰理气方及其拆方改善细颗粒物肺损伤的机制研究[J].世界中医药,2025,20(14):2458-2467,10.基金项目
国家自然科学基金项目(82074345) （82074345）
