世界中医药2025，Vol.20Issue(14)：2468-2472,2480,6.DOI:10.3969/j.issn.1673-7202.2025.14.009
茯苓多糖对肺腺癌A549细胞生物学行为的影响
Effects of Poria cocos Polysaccharides on Biological Behaviors of Lung Adenocarcinoma A549 Cells
摘要
Abstract
Objective:To investigate the effects of Poria cocos polysaccharides(PCP)on the biological behaviors of lung adenocar-cinoma A549 cells and the Janus kinase-1(JAK1)/signal transducer and activator of transcription 3(STAT3)signaling pathway in non-small cell lung cancer(NSCLC).Methods:The effect of PCP on A549 cell viability was assessed using the methyl thiazolyl tetrazolium(MTT)assay to determine subsequent experimental concentrations.Cell proliferation,migration,invasion,and apoptosis were evaluated using colony formation,scratch,Transwell assays,and flow cytometry,respectively.The mRNA levels of proliferation-related genes were measured by PCR,and protein expression levels associated with migration,invasion,apoptosis,and the JAK1/STAT3 pathway were detected by Western blot.Results:Treatment with PCP at 30,40,and 50 mg/mL significantly reduced A549 colony formation rate,scratch closure rate,number of invading cells,and expression levels of cyclin D1 mRNA,c-Myc mRNA,N-cadherin,vimentin,matrix metalloproteinase-2(MMP-2),MMP-9,Bcl-2,phosphorylated JAK1(p-JAK1),and phosphorylated STAT3(p-STAT3)compared with the control(0 mg/mL PCP)(P＜0.05).In contrast,apoptosis rate and protein levels of E-cad-herin,cleaved caspase-3,and Bax were significantly increased(P＜0.05),exhibiting dose-dependent effects(P＜0.05).Conclu-sion:PCP inhibits the malignant biological behaviors of A549 cells by downregulating JAK1/STAT3 signaling activity and modula-ting cell proliferation,migration,and invasion.关键词
非小细胞肺癌/肺腺癌/茯苓多糖/细胞增殖/细胞迁移/细胞侵袭/细胞凋亡/JAK1/STAT3Key words
Non-small cell lung cancer/Lung adenocarcinoma/Poria cocos polysaccharides/Cell proliferation/Cell migration/Cell invasion/Apoptosis/JAK1/STAT3分类
医药卫生引用本文复制引用
潘纯钰,白斌,韩永建,杨媛媛,郭晓萱..茯苓多糖对肺腺癌A549细胞生物学行为的影响[J].世界中医药,2025,20(14):2468-2472,2480,6.基金项目
国家自然科学基金面上项目(82374148) （82374148）
河南省科技厅科技攻关项目(182102311129) （182102311129）
