生物技术通报2025，Vol.41Issue(10)：32-42,11.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0506
PPR蛋白调控叶绿体RNA编辑分子机制研究进展
Research Progress in the Molecular Mechanism of PPR Protein-regulated Chloroplast RNA Editing
摘要
Abstract
As semi-autonomous organelles,chloroplasts possess their own genomes,and some of their transcribed RNAs require C-to-U base changes to ensure correct gene expression.PPR(pentatricopeptide repeat protein)proteins are the core regulatory factors controlling chloroplast RNA editing.This family is large and is divided into two subfamilies,P-type and PLS-type,based on their different C-terminal domains.The PLS-type subfamily can be further classified into PLS-E,PLS-E+,and PLS-DYW.Among them,the DYW domain in the PLS-DYW type has deaminase activity and directly participates in RNA editing.The PPR motif,which is unique to PPR proteins,recognizes the RNA sequence upstream of the editing site in the 5'-3' direction based on the combination of the 6th and 1st amino acids from the N-terminal to the C-terminal.This modular recognition mechanism enables PPR proteins to screen editing sites in a one-PPR-motif-to-one-nucleotide manner,recruit and assemble the editing complex,and carry out the editing process.Defects in RNA editing regulated by related PPR proteins can lead to abnormal chloroplast development,causing plant wilting or death.This article reviews the current research progress,focusing on the molecular mechanisms by which PPR proteins regulate RNA editing of chloroplast genes in different plants,and prospects to the dynamic assembly process of the RNA editing complex,providing references for future in-depth exploration of the targeting mechanism of PPR proteins and their applications in agriculture.关键词
叶绿体/PPR蛋白/RNA编辑/光合作用Key words
chloroplast/PPR proteins/RNA editing/photosynthesis引用本文复制引用
李新颖,孙晶,吕若彤,任亚娟,罗蕾,艾鹏飞,王雁伟..PPR蛋白调控叶绿体RNA编辑分子机制研究进展[J].生物技术通报,2025,41(10):32-42,11.基金项目
河北省高等学校科学研究项目(BJK2024165),河北科技大学引进人才科研启动基金(QD2023010) （BJK2024165）
