生物技术通报2025，Vol.41Issue(10)：72-86,15.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0620
运用小麦杂交株系挖掘环式光合电子传递调控基因并应用于作物高光效改造
Utilizing Wheat Hybrid Lines to Mine Genes Regulating Cyclic Electron Flow and Applying Them in Improving Photosynthetic Efficiency in Crops
摘要
Abstract
[Objective]Global warming and a rapidly increasing global population present formidable challenges to food security,necessitating significant advancements in both crop yield improvement and enhanced resilience to environmental stresses.Photosynthesis serves as the foundation of crop productivity,with the cyclic electron flow during the light reactions coupling ATP synthesis and modulating reducing power accumulation.This pathway plays an important role in heat adaptation and photosynthetic efficiency,making the identification and application of its genetic regulators imperative.[Method]This study utilized representative wheat lines with varying cyclic photosynthetic electron transport activities as references,combined with photosynthetic parameter measurements,protein content assays,and transcriptome sequencing techniques.From the recombinant inbred lines derived from the crossbreeding of wheat varieties Xiaoyan 54 and Jing 411,strains exhibiting polar separation in cyclic electron transport activity were screened and differentially expressed genes were obtained.[Result]The strains with higher cyclic electron transport activity also demonstrated elevated linear electron transport activity and photosynthetic CO2 assimilation rates,maintaining these advantages under increased light intensity,making them suitable for application as high-efficiency breeding materials.Through differential gene expression analysis,a series of functional genes and transcription factors with potential to enhance cyclic photosynthetic electron transport or overall photosynthetic activity were identified.Selected genes(including TaPnsL2 and TaNAC)were constructed into overexpressing vectors and transformed into the rice cultivar Xiushui 134.The T1 and T2 generation transgenic materials presented advantages in photosynthetic rate during field trials in both Hainan province and Shanghai.[Conclusion]Through genetic screening or modification targeting the activity of cyclic electron transport,there is potential to enhance the photosynthetic efficiency or adaptability to high light intensity in crops.关键词
环式电子传递/光合作用/NDH复合体/遗传调控/小麦/水稻Key words
cyclic electron transport/photosynthesis/NDH complex/genetic regulation/wheat/rice引用本文复制引用
范艳飞,叶露幻,李雨桐,王钏跞,张瑞,罗建华,王鹏..运用小麦杂交株系挖掘环式光合电子传递调控基因并应用于作物高光效改造[J].生物技术通报,2025,41(10):72-86,15.基金项目
农业生物育种国家科技重大专项(2023ZD04072) （2023ZD04072）
