生物技术通报2025，Vol.41Issue(10)：87-97,11.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0550
叶绿体中过表达乙醇酸氧化酶对水稻光合作用与生长的影响
Expression of Glycolate Oxidase in Rice Chloroplasts and Its Effects on Photosynthesis and Growth
摘要
Abstract
[Objective]Validate the hypothesis that plant chloroplasts possess the ability to metabolize glyoxylate into CO₂.[Method]In this study,the rice glycolate oxidase 1(OsGLO1),glycolate oxidase 3(OsGLO3)and catalase C(OsCATC)genes and the catalase(EcKAT)gene from Escherichia coli were cloned,and the chloroplast localization signal coding sequence RC2 was fused to their front ends;subsequently,GLO and CAT/KAT were combined in different combinations to construct different multi-gene expression vectors,which were directed its expression in rice chloroplasts,thereby catalyzing the production of glyoxylic acid from glycolic acid in chloroplasts.[Result]Results from transcript-level,protein-level,and enzyme activity analyses confirmed the high-efficiency expressions of target genes in rice and having normal catalytic function.However,all transgenic rice plants had phenotypes such as dwarfism,reduced tiller number,and decreased photosynthesis.[Conclusion]These findings indicate that only converting glycolate into glyoxylate in chloroplasts fails to enhance photosynthetic carbon fixation efficiency in rice,suggesting that rice chloroplasts may lack the capacity to catalyze glyoxylate into CO₂.关键词
乙醇酸氧化酶/过氧化氢酶/乙醛酸/叶绿体/光合作用/CO2/水稻/光呼吸Key words
glycolate oxidase/catalase/glyoxylate/chloroplasts/photosynthesis/CO2/rice/photorespiration引用本文复制引用
李波娣,李志超,朱国辉,彭新湘,张智胜..叶绿体中过表达乙醇酸氧化酶对水稻光合作用与生长的影响[J].生物技术通报,2025,41(10):87-97,11.基金项目
广东省自然科学基金项目(2025A1515012660) （2025A1515012660）
