实用医学杂志2025，Vol.41Issue(20)：3155-3164,10.DOI:10.3969/j.issn.1006-5725.2025.20.003
氯沙坦对大脑蓝斑核中血管紧张素Ⅱ所诱导的高盐摄入的影响
Effect of losartan on high salt intake induced by angiotensin Ⅱ in the locus coeruleus of the brain
摘要
Abstract
Objective By observing the effect of angiotensin Ⅱ in the locus coeruleus of the brain on high salt intake and the impact of losartan on this effect,this study explores effective implementation methods for salt restriction strategies.Methods Brain catheterization and microinjection were used to administer single microinjection of Ang Ⅱ into the locus coeruleus of rats,as well as combined microinjection of saline,Ang Ⅱ,and angiotensin Ⅱ type 1 receptor,AT1R antagonist losartan was used to observe the changes in the intake and water intake of hypertonic sodium chloride solution in rats with different sodium intake models;Single microinjection of Ang Ⅱ into the locus coeruleus of rats was performed to observe changes in water intake in water deprived rats,as well as changes in urine output,sodium excretion,horizontal and vertical activity in normal rats.Results Whether in rats treated with"water deprivation partial rehydration(WD-PR)"or in rats treated with"subcutaneous combined injection of furosemide(FURO)and Captopril(CAP)(FURO-CAP)",microinjection of 0.1 ng,1 ng,and 10 ng doses of Ang Ⅱ into the locus coeruleus caused a dose-dependent increase in 0.3 mol/L NaCl intake and water intake.However,pre injection of AT1R antagonist losartan at doses of 0.5 μ g,5 μ g,and 50 μ g significantly inhibited the injection of 10 ng Ang Ⅱ into the same site in a dose-dependent manner.The increase in intake of 0.3 mol/L NaCl and water caused by it.Compared with injection of saline into the locus coeruleus,injection of 10.0ng dose of Ang Ⅱ into the locus coeruleus significantly increased the horizontal and vertical activity of rats,but had no significant effect on renal excretion.Conclusion Losartan can inhibit the high salt intake induced by Ang Ⅱ in the locus coeruleus of the brain,and can be used as an effective drug in the salt limiting strategy for controlling hypertension.关键词
蓝斑核/血管紧张素Ⅱ/氯沙坦/高盐摄入/肾排泄/活动度Key words
locus coeruleus/Ang Ⅱ/losartan/high sodium intake/renal excretion/activity level分类
医药卫生引用本文复制引用
闫君宝,陈义祥,牛小艳,陆兆丰..氯沙坦对大脑蓝斑核中血管紧张素Ⅱ所诱导的高盐摄入的影响[J].实用医学杂志,2025,41(20):3155-3164,10.基金项目
国家自然科学基金项目(编号:82170133) （编号:82170133）
河南省高等学校重点科研项目(编号:22A310018) （编号:22A310018）
