实用医学杂志2025，Vol.41Issue(20)：3165-3174,10.DOI:10.3969/j.issn.1006-5725.2025.20.004
金钗石斛总生物碱对TGF-β2诱导的人晶状体上皮细胞增殖、迁移及上皮间质转化的调控作用
The regulatory role of Dendrobium nobile Lindl alkaloids on the proliferation,migration,and epithelial-mesenchymal transition of human lens epithelial cells induced by TGF-β2
摘要
Abstract
Objective To investigate the regulatory effect of Dendrobium nobile Lindl alkaloids(DNLA)on the proliferation,migration,and epithelial-mesenchymal transition(EMT)of HLEB3 cells induced by TGF-β2.Methods HLEB3 cells were cultivated in vitro and classified into the control group(DZ),the model group(TGF-β2),and the treatment group(TGF-β2+DNLA).TGF-β2 induced the EMT process of HLEB3 cells.Changes in cell morphology were observed through an inverted microscope.Cell proliferation,apoptosis,and migration were detected by CCK-8 assay,scratch test,and Transwell assay.The total RNAs of the samples were extracted for transcriptome analysis.Bioinformatics processing was employed to obtain relevant information on gene expression differences at the transcriptional level,biological processes,and related signaling pathways.The Western blot(WB)technique was utilized to detect EMT-related proteins to elucidate the mechanism of action of DNLA on lens epithelial cells.Results The study revealed that 10 μg/ml DNLA was suitable for the growth of HLEB3 cells.After 48 hours of the scratch test,the migration rate of the TGF-β2+DNLA group significantly decreased(P<0.01).The Transwell results indicated that the cell migration ability of the TGF-β2+DNLA group was notably weakened(P<0.05).Through bioinformatics,it was discovered that the prevention and treatment of posterior capsular opacification(PCO)by DNLA might be associated with cell junctions,cytoskeleton construction,and fibronectin binding.The pathogenesis of PCO may be related to multiple signaling pathways,including the TGF-β signaling pathway,TNF signaling pathway,and PI3K-Akt signaling pathway.Simultaneously,DNLA could reduce the expression levels of FNI,Smad2/3,and α-SMA proteins and increase the expression of E-cadherin protein.This indicates that DNLA can alleviate abnormal proliferation,migration,and EMT of lens epithelial cells by enhancing intercellular adhesion junctions and weakening cell migration ability,thereby playing a role in preventing and treating posterior capsular opacification.Conclusions DNLA can significantly inhibit the abnormal proliferation and migration of HLEB3 cells,alleviate the EMT process induced by TGF-β2,and prevent and control the occurrence of posterior capsular opacification and other ocular diseases.The mechanism of action might be related to the intervention of the TGF-β/smad signaling pathway and fibrosis proteins such as ZO-1 and E-cadherin.关键词
金钗石斛总生物碱/上皮间质转化/晶状体上皮细胞损伤/α-平滑肌肌动蛋白Key words
Dendrobium nobile Lindl alkaloids/epithelial-mesenchymal transition/lens epithelial cell injury/α-SMA分类
医药卫生引用本文复制引用
时应兰,黄朝霞,汤小芳,石谍,陈静,李军..金钗石斛总生物碱对TGF-β2诱导的人晶状体上皮细胞增殖、迁移及上皮间质转化的调控作用[J].实用医学杂志,2025,41(20):3165-3174,10.基金项目
贵州省科技计划项目(编号:黔科合基础-ZK[2021]一般427) （编号:黔科合基础-ZK[2021]一般427）
贵州中医药大学国家与省级科技创新人才团队培育项目(编号:贵中医TD合字[2024]002号,贵中医博士启动基金[2020]58号) （编号:贵中医TD合字[2024]002号,贵中医博士启动基金[2020]58号）
