时珍国医国药2025，Vol.36Issue(20)：3846-3852,7.DOI:10.70976/j.1008-0805.SZGYGY-2025-2007
补肾养营活血方抑制子宫内膜细胞焦亡预防TCRA术后宫腔粘连的研究
Bushen Yangying Huoxue Formula(补肾养营活血方)preventing the recurrence of intrauter-ine adhesions via inhibiting endometrium cells pyroptosis
摘要
Abstract
Objective To explore the intervening effect and mechanism of Bushen Yangying Huoxue Formula(补肾养营活血方,BYHF)on intrauterine adhesions(IUA)rats with kidney deficiency and blood stasis.Methods A total of 24 adult female Sprague-Dawley(SD)rats were randomized into the Control group,Model group,and BYHF group,with 8 rats each.A rat model of IUA with kidney deficiency and blood stasis was established through mechanical damage,subcutaneous injection of epinephrine,and intragastric administration of hydroxyurea solution.The rats in the BYHF group were administered BYHF intragastrically(2.943g·kg-1·d-1)for 3 weeks and those from the Control and Model groups were administered the same volume of distilled water.Tissue samples were collected from anesthetized rats in estrous cycles.The syndrome of kidney deficiency(evaluated by cAMP,cAMP/cGMP)and blood stasis(evalu-ated by ET,TXB2)were assessed by ELISA.The thickness of the endometrium,number of glands and fibrosis rate were evaluated using HE and Masson staining,respectively.Western blot(WB)was utilized to detect the protein levels of NLRP3,Caspase-1,and IL-18.Human endometrial stromal cells(HESCs)were induced by LPS and Nigericin to establish in vitro pyroptosis models.BYHF-containing serum was used to culture HESCs.Pyroptotic cells were visualized via staining with Calcein AM/PI.The concentrations of Caspase-1,IL-1β and IL-18 in the supernatants were assessed by ELISA.Results BYHF was able to alleviate the syndrome of kidney deficiency and blood stasis of IUA(P<0.01,P<0.05),reduce the degree of fibrosis(P<0.01),improve the thickness of the endome-trium and the number of glands(P<0.05),and downregulate the expressions of NLRP3(P<0.01),Caspase-1(P<0.01),and IL-18(P<0.05).BYHF-containing serum inhibited pyroptosis of HESCs and the expression of the pyroptosis-related factors(Caspase-1,IL-1β and IL-18)(P<0.05).Conclusion BYHF could alleviate the syndrome of kidney deficiency and blood stasis,and inhibit pyroptosis for preventing IUA and promoting endometrial regeneration.关键词
补肾养营活血方/细胞焦亡/宫腔粘连/NLRP3/Caspase-1/IL-18通路Key words
Bushen Yangying Huoxue Formula(补肾养营活血方)/Pyroptosis/Intrauterine adhesions(IUA)/NLRP3/Cas-pase-1/IL-18分类
医药卫生引用本文复制引用
陈琇涵,杨茜,李方远..补肾养营活血方抑制子宫内膜细胞焦亡预防TCRA术后宫腔粘连的研究[J].时珍国医国药,2025,36(20):3846-3852,7.基金项目
中国博士后科学基金面上资助项目(2022M722295) （2022M722295）
国家自然科学基金青年基金(82305302) （82305302）
四川省重点研发项目(2024YFFK0167) （2024YFFK0167）
