时珍国医国药2025，Vol.36Issue(20)：3868-3873,6.DOI:10.70976/j.1008-0805.SZGYGY-2025-2010
肾脑复元汤通过Mir-210/BCL-2/Beclin-1轴调控缺血性脑卒中细胞自噬的作用机制
Shennao Fuyuan Decoction(肾脑复元汤)regulates autophagy in ischemic stroke via the Mir-210/BCl-2/Beclin-1 axis
摘要
Abstract
Objective To investigate the effect of Shennao Fuyuan Decoction(肾脑复元汤,SNFYD)on cellular autophagy after isch-emic injury in focal cerebral ischemia rats and its regulatory role in cerebral ischemia.Methods A rat model of focal cerebral ischemia(MCAO)was established.Thirty-six male SD rats were randomly divided into three groups:the blank control group,model group,and SNFYD group.Tissues were harvested after 3 days of administration.Brain tissue structure was observed by HE staining.The expres-sions of microtubule-associated protein 1 light chain 3(LC3),beclin-1,and B-cell lymphoma-2(BCL-2)were detected by Western blot(WB).LC3 expression was detected by immunofluorescence.The mRNA expressions of LC3,Beclin-1,BCL-2,and microRNA-210(Mir-210)were measured by RT-qPCR.Results Compared with the blank control group,the model group showed increased patho-logical damage,decreased BCL-2 expression(P<0.01),upregulated LC3 and Beclin-1 protein and mRNA levels,and elevated Mir-210 mRNA expression(P<0.01).In contrast,when compared with the model group,the SNFYD group exhibited alleviated tissue damage,downregulated LC3,Beclin-1,and Mir-210 expressions,and upregulated BCl-2 expression(P<0.01).Conclusion SNFYD may exert a protective effect on brain tissue by inhibiting Mir-210 levels,downregulating Beclin-1,upregulating BCL-2,and suppress-ing cellular autophagy.关键词
肾脑复元汤/缺血性脑卒中/局灶性脑缺血/细胞自噬/MicroRNA-210/Beclin1蛋白Key words
Shennao Fuyuan Decoction(肾脑复元汤)/Schemia stroke/Focal Cerebral Ischemia/Autophagy/MicroRNA-210/Beclin-1 protein分类
医药卫生引用本文复制引用
刘侃,邹婷,任木千子,胡国恒,唐路军,曾阳,邱华安..肾脑复元汤通过Mir-210/BCL-2/Beclin-1轴调控缺血性脑卒中细胞自噬的作用机制[J].时珍国医国药,2025,36(20):3868-3873,6.基金项目
湖南省教育厅科学研究项目(20C1420) （20C1420）
湖南中医药大学中医学院院级项目(07号) （07号）
湖南中医药大学校级课题(2022XYLH010) （2022XYLH010）
湖南省自然科学基金(2024JJ9429) （2024JJ9429）
湖南省自然科学基金(2025JJ80979) （2025JJ80979）
