时珍国医国药2025，Vol.36Issue(20)：3882-3889,8.DOI:10.70976/j.1008-0805.SZGYGY-2025-2012
连吴茱萸炮制前后的高效液相指纹图谱及色度值差异研究
Comparative study on HPLC fingerprint and color value of Fructus Evodiae before and after processing with Rhizoma Coptidis
摘要
Abstract
Objective To establish HPLC fingerprints and measure color values to compare the differences between Fructus Evodiae(FE)and Fructus Evodiae processed with Rhizoma Coptidis(RC-FE).Methods Differences between FE and RC-FE were analyzed using similarity evaluation,paired sample t-test,principal component analysis(PCA),and orthogonal partial least squares-discriminant analysis(OPLS-DA).The correlation between color values and chemical constituents was also analyzed.Results The HPLC fingerprints revealed 20 and 22 common peaks in FE and RC-FE,respectively,with 10 components identified.New compo-nents,palmatine hydrochloride and berberine,were detected after processing.Peaks 1,2,3,6,8,16(palmatine hydrochloride),and 17(berberine)were the main differential constituents between FE and RC-FE.The peak areas of peaks 1,2,3,6,and 8 decreased significantly after processing compared to before processing.The ΔE*ab values of CR-FE ranged from 2.83 to 12.73.The peak areas of peaks 4(neochlorogenic acid),9(chlorogenic acid),and 10(cryptochlorogenic acid)showed significant positive correlations with E*ab,whereas those of peaks 12(narcissoside),16(palmatine hydrochloride),17(berberine),20(evodiamine),and 21(rutae-carpine)showed significant negative correlations with E*ab.Conclusion Both the chemical composition and color of RC-FE changed after processing with Rhizoma Coptidis,providing a reference for the quality evaluation and the significance of processing of this prepared herbal medicine.关键词
连吴茱萸/炮制/色度值/高效液相指纹图谱/差异性成分Key words
Fructus Evodiae processed with Rhizoma Coptidis/Processing/Color value/HPLC fingerprint/Differential com-ponents分类
医药卫生引用本文复制引用
林晓彤,蒙已钦,曾春晖,刘雯,农丽惠,黎海凤,刘舒凌..连吴茱萸炮制前后的高效液相指纹图谱及色度值差异研究[J].时珍国医国药,2025,36(20):3882-3889,8.基金项目
广西自然科学基金(2025GXNSFAA069558) （2025GXNSFAA069558）
广西中医药大学校级科研项目(2023MS019) （2023MS019）
国家中医药管理局"中药炮制传承技术传承基地"资助项目[桂中医药发(2022)9号] （2022）
广西壮族自治区中医药重点研究室建设项目[桂中医药科教发(2023)9号] （2023）
广西中医药大学赛恩斯新医药学院大学生创新创业训练项目(自治区级,S202413643059) （自治区级,S202413643059）
