时珍国医国药2025，Vol.36Issue(20)：3890-3895,6.DOI:10.70976/j.1008-0805.SZGYGY-2025-2013
半夏试管块茎和试管苗生长发育的特征特性研究
Study on the characteristics and developmental features of in vitro tubers and plantlets of Pinellia ternata(Thunb.)Breit
摘要
Abstract
Objective To investigate the changes in morphology,starch granules,and starch enzyme activity during multiple shoot emergence from in vitro tubers of Pinellia ternata(Thunb.)Breit.(PT),providing a theoretical basis for efficient regulation of tissue-cultured tubers of PT and artificial seed tuber production.Methods Morphological indexes derived from 1-5 generations of tissue-cultured tubers of PT were measured.Paraffin sectioning techniques were employed to study changes in starch enzyme activity and starch granule distribution/accumulation in vitro tubers.Results With increasing generation number,plants grew taller and tubers increased in size.Third-generation tubers produced plants with trifoliolate leaves and bulbils.Activities of sucrose synthase(SS),sucrose phosphate synthase(SPS),and starch phosphorylase(SP)peaked in third-generation tubers(initial increase followed by decrease).Starch granule number showed similar trend,with highest accumulation in third-generation tubers.Starch granule volume increased with generations.Conclusion Tissue-cultured tubers of PT exhibit morphology and growth characteristics similar to natural PT,validating their use as artificial seed tubers.Due to maturation during shoot emergence cycles,vigorously vital second-generation in vitro tubers are optimal for production.关键词
半夏/试管块茎/多次抽苗/生长发育/淀粉Key words
Pinellia ternata(Thunb.)Breit./In vitro tuber/Multiple shoot emergence/Growth and development/Starch分类
农业科技引用本文复制引用
王进秀,樊慧,张延红,何春雨,郭清毅,王琳佳,徐子杰..半夏试管块茎和试管苗生长发育的特征特性研究[J].时珍国医国药,2025,36(20):3890-3895,6.基金项目
甘肃省联合基金项目(24JRRA877) （24JRRA877）
甘肃省重点研发计划项目(24YFNA007) （24YFNA007）
甘肃省高等学校产业支撑计划项目(2020C-09/2024CYZC-40) （2020C-09/2024CYZC-40）
