塔里木大学学报2025，Vol.37Issue(5)：9-18,10.DOI:10.3969∕j.issn.1009-0568.2025.05.002
基于形态学和高通量测序技术的巴里坤湖浮游生物多样性比较研究
Comparative study on the diversity of plankton in Barkol Lake based on morphology and high-throughput sequencing
摘要
Abstract
High-throughput sequencing technology has been widely applied to biodiversity surveys of common water bodies such as freshwater and seawater,but its application in salt lake plankton biodiversity monitoring remains relatively scarce.This study,taking Barkol Lake in Xinjiang as the research object,jointly employed high-throughput sequencing and morphological identification to conduct a comparative analysis of plankton biodiversity.Results showed:both methods collectively identified plankton from 10 phyla and 110 genera,with 7 phyla and 14 genera being commonly identified.For cyanobacteria(prokaryotic plankton),66.67%of the species identified by morphological characteristics could be detected by 16S rDNA sequencing,while 89.66%of the species obtained through 16S rDNA sequencing could not be identified by morphological methods.For eukaryotic plankton,only 14.04%of the morphologically identified species could be detected by 18S rDNA sequencing,and 63.64%of the species obtained through 18S rDNA sequencing could not be identified morphologically.Both methods showed consistency in identifying dominant species.High-throughput sequencing technology demonstrates clear advantages in plankton taxonomic composition and relative abundance identification,with high efficiency and low cost,though some taxa remain unidentifiable;morphological identification is time-consuming and prone to overlooking small species.Therefore,it is recommended to combine high-throughput sequencing and morphological identification techniques to comprehensively and accurately reveal salt lake plankton biodiversity.关键词
形态学鉴定/高通量测序技术/浮游生物/16S rDNA/18S rDNAKey words
morphology/high-throughput sequencing/plankton/16 S rDNA/18 S rDNA分类
生物科学引用本文复制引用
刘昌财,韩家难,王智超,王晨悦,史楠楠,穆罕默德·萨利姆·常..基于形态学和高通量测序技术的巴里坤湖浮游生物多样性比较研究[J].塔里木大学学报,2025,37(5):9-18,10.基金项目
国家自然科学基金地区科学基金项目(32260299) （32260299）
