武汉工程大学学报2025，Vol.47Issue(5)：531-538,8.DOI:10.19843/j.cnki.CN42-1779/TQ.202404001
稳定性同位素探针技术及其微生物生态学应用
DNA-based stable isotope probing and its application in microbial ecology
摘要
Abstract
DNA-based stable isotope probing(DNA-SIP)is an emerging technique developed over the past two decades that combines the strengths of isotope tracing and molecular biology,enabling the isolation and identification of metabolically active microorganisms in complex environments.This review first outlines the fundamental principles of DNA-SIP,including the selection of isotope-labeled substrates,cultivation conditions,and downstream molecular analysis methods,and highlights the importance of advances in high-throughput sequencing and bioinformatics for improving resolution and sensitivity.Building on this foundation,we summarized recent applications of DNA-SIP in microbial ecology:(i)revealing key functional groups involved in biogeochemical cycles of carbon,nitrogen,and sulfur;(ii)elucidating plant-microbe interactions in the rhizosphere and their contribution to nutrient acquisition;and(iii)identifying major degraders of organic pollutants in contaminated environments.Despite challenges such as cross-feeding,differential isotope incorporation,and complex data analysis,the integration of DNA-SIP with metagenomics,single-cell Raman spectroscopy,and other emerging approaches is expected to further expand its utility.Overall,DNA-SIP provides a powerful and unique tool for disentangling microbial functions in situ and holds broad prospects for advancing research in environmental science and microbial ecology.关键词
同位素/稳定性同位素核酸探针/微生物功能Key words
stable isotope/DNA-based stable isotope probing(DNA-SIP)/microbial function分类
农业科技引用本文复制引用
余心洁,樊昊心..稳定性同位素探针技术及其微生物生态学应用[J].武汉工程大学学报,2025,47(5):531-538,8.基金项目
国家自然科学基金(4207039) （4207039）
