摘要
Abstract
Objective To construct a mouse sleep deprivation(SD)model by using sleep disturbance and increasing light at night(LAN)exposure,and to analyze the possible mechanism of SD on the onset of cor-onary artery disease(CAD).Methods Twelve 2-month-old male ICR mice were selected,they were randomly divided into SD model group and control group,with 6 rats in each group.The SD model of adult ICR mice was constructed..Through public database retrieval,the genes that may be involved in CAD were screened.Real-time quantitative reverse transcription PCR(qRT-PCR)was used to verify the differences in mRNA levels of the screened genes,and Western blot was further used to verify the protein expression characteristics of the related molecules.Results Ten CAD-related genes were screened through published literature and public da-tabase searches.The qRT-PCR results showed that there were significant differences in mRNA expression of five genes,including proprotein convertase subtilisin/kexin type 9(PCSK9),mitogen activated protein kinase 5(MAPKAPK5),Tectonic1(TCTN1),Kaptin(KPTN),and regulatory of G protein signaling 19(RGS19),between the control group and the SD model group(P<0.05).Further Western blot results confirmed that the expression patterns of PCSK9,MAPKAPK5,TCTN1,KPTN,and RGS19 proteins were consistent with their mRNA changes.In addition,the protein level of inflammatory factors(IL-6,TNF-α)were found signifi-cantly increased in the myocardial tissue of SD group mice(P<0.05).Conclusion There are differences in the expression of CAD-related genes such as PCSK9,MAPKAPK5,TCTN1,KPTN and RGS19 in mouse SD model,which may be involved in the pathogenesis of SD-related CAD.关键词
冠心病/睡眠剥夺/光暴露/差异表达基因/炎症因子Key words
Coronary artery disease/Sleep deprivation/Light exposure/Differential expressed genes/Inflammatory cytokines分类
医药卫生
