现代医药卫生2025，Vol.41Issue(10)：2326-2331,6.DOI:10.3969/j.issn.1009-5519.2025.10.014
不同时效重组人生长激素对矮小症儿童肠道菌群的影响
Effect of recombinant human growth hormone of different timings on the intestinal flora of children with short stature
摘要
Abstract
Objective To explore the effects of recombinant human growth hormone(rhGH)on the composition and richness of intestinal flora in children with short stature.Methods A longitudinal follow-up of 6 months was conducted on 32 children with short stature aged 3 to<14 years who were treated with rh-GH for the first time at Subei People's Hospital Affiliated to Yangzhou University from July 2021 to June 2022.According to the different timeliness of rhGH,they were divided into long-acting group(group L,n=16)and short-acting group(group S,n=16).Group L was treated with polyethylene glycol recombinant human growth hormone(PEG-rhGH)injection,and group S was treated with rhGH injection.Fecal samples were collected before rhGH treatment,at 3 months and 6 months of treatment for high-throughput sequencing and bioinformatics analysis.Anosim and LEfSe analysis were used to compare the differences in the structure and richness of the intestinal microbiota community in children during different treatment periods.The correlation between intestinal flora abundance and growth rate(GV)and insulin-like growth factor-1(IGF-1)was ana-lyzed.Results Finally,83 fecal specimens were collected.The results of repeated-measures ANOVA showed that the differences in the time effect,between-group effect,and interaction effect of GV were statistically sig-nificant(F=93.402,P<0.001;F=4.354,P=0.046;F=5.593,P=0.011),whereas only the time effect of IGF-1 was statistically significant(F=84.393,P<0.001).After simple effects analysis using the Bonferroni method corrected for the test level of α=0.05(α=0.004),the GV of group S and group L at 3 months[(0.78±0.27),(0.78±0.29)cm/month]and 6 months[(0.83±0.18),(1.11±0.26)cm/month]were higher than those before treatment[(0.29±0.06),(0.26±0.03)cm/month],and the differences were statis-tically significant(P<0.004).There was no significant difference in GV between 3 months and 6 months af-ter treatment in group S(P>0.004),while there was significant difference in GV between 3 months and 6 months after treatment in group L(P<0.004).At 6 months of treatment,there was a statistically significant difference in GV between group S and group L(P<0.004).The levels of IGF-1 in group S and group L at 3 months[(213.22±80.97),(183.19±53.02)ng/mL]and 6 months[(346.46±78.86),(354.97±115.40)ng/mL]were higher than those before treatment[(129.41±71.01),(111.82±44.17)ng/mL],and the level of IGF-1 at 6 months of treatment was higher than that at 3 months of treatment,the differences were statisti-cally significant(P<0.004).There was no significant difference in IGF-1 level between group S and group L at different follow-up stages(P>0.004).The results of Anosim analysis showed that there were significant differences in the microbial community structure of children in group L at any time before treatment,3 months after treatment and 6 months after treatment(R=0.102,P=0.030;R=0.176,P=0.025;R=0.298,P=0.004);in the group S,the difference were statistically significant at 3 and 6 months of treatment compared with those before treatment(R=0.170,P=0.004;R=0.202,P=0.014).Species difference analy-sis showed that the difference species in group L was enterococcus at 6 months of treatment,and the difference species in group S was verrucomicrobiota before treatment.Correlation analysis showed that IGF-1 was posi-tively correlated with the abundance of Bacteroides phylum(genus)in both group L and group S after 6 months of treatment(r=0.637,0.577,P<0.05),and was positively correlated with the abundance of Bifidobacterium genus in group L after 3 months of treatment(r=0.562,P<0.05).GV was negatively cor-related with the abundance of Actinomycota and Proteobacteria in group S after 3 months of treatment(r=-0.565,-0.500,P<0.05),and negatively correlated with the abundance of Proteobacteria in group L after 6 months of treatment(r=-0.702,P<0.05).Conclusion rhGH can increase the abundance of beneficial bacteria by regulating the richness and diversity of intestinal flora,and the effect of PEG-rhGH is more obvi-ous than that of rhGH.关键词
重组人生长激素/肠道菌群/矮小症/儿童/生长Key words
Recombinant human growth hormone/Intestinal flora/Short stature/Children/Growth分类
医药卫生引用本文复制引用
经纬,陈文雅,马春时,解淑钰,陶月红..不同时效重组人生长激素对矮小症儿童肠道菌群的影响[J].现代医药卫生,2025,41(10):2326-2331,6.基金项目
长春金赛药业横向课题(HX2021012). （HX2021012）
