现代医药卫生2025，Vol.41Issue(10)：2366-2370,5.DOI:10.3969/j.issn.1009-5519.2025.10.021
外泌体miR-20a、miR-21表达与慢性乙型肝炎病情进展的关联研究
The correlation between the expression of exosomal miR-20a and miR-21 and the progression of chronic hepatitis B
摘要
Abstract
Objective To analyze the correlation between the expression of exosomal microRNA-20a(miR-20a),microRNA-21(miR-21)and the progression of chronic hepatitis B(CHB).Methods A retrospec-tive study was conducted.The subjects were 135 CHB patients admitted to Kaifeng Third People's Hospital from October 2021 to April 2024.According to the different stages of disease progression,80 patients with CHB in immune tolerance stage were classified as early group,and 55 patients with CHB in immune activation stage were classified as progressive group.The differences of exosomal miR-20a,miR-21,functional factors of myeloid-derived suppressor cells(MDSCs)[arginase 1(ARG1),nitric oxide(NO),inducible nitric oxide syn-thase(iNOS)]and liver function indexes[alanine transaminase(ALT),aspartate transaminase(AST),alka-line phosphatase(ALP),total bilirubin(TBil)]were compared between the two groups.Pearson correlation coefficient was used to test the correlation between miR-20a,miR-21 and MDSCs functional factors,liver func-tion indicators.The predictive value of miR-20a and miR-21 on the progression of CHB patients were verified by drawing the receiver operating characteristic(ROC)curve.Results The levels of miR-20a,miR-21,AGR1,NO and iNOS in the early group were higher than those in the progressive group,and the levels of ALT,AST,ALP and TBil were lower than those in the progressive group,the differences were statistically significant(P<0.05).Pearson correlation coefficient test showed that miR-20a was positively correlated with AGR1,NO and iNOS(P<0.05),and negatively correlated with ALT,AST,ALP and TBil(P<0.05).MiR-21 was positively correlated with AGR1,NO and iNOS(P<0.05),and negatively correlated with ALT,AST,ALP and TBil(P<0.05).The ROC curve showed that the sensitivity and specificity of miR-20a in predicting the progression of CHB patients were 81.25%and 78.83%,respectively,and the sensitivity and specificity of miR-21 in predicting the progression of CHB patients were 80.33%and 75.45%,respectively[area under the curve(AUC)=0.863,0.862].Conclusion High expression of miR-20a and miR-21 can inhibit the immune function of CHB patients by activating the function of MDSCs and aggravate their condition.Therefore,moni-toring the changes of miR-20a and miR-21 levels can realize the dynamic evaluation of their disease progres-sion and guide clinical decision-making.关键词
慢性乙型肝炎/髓源性抑制性细胞/肝功能损伤/微小RNA-20a/微小RNA-21Key words
Chronic hepatitis B/Myeloid inhibitory cells/Liver function injury/MicroRNA-20a/MicroRNA-21分类
医药卫生引用本文复制引用
田金茹,王孟丽..外泌体miR-20a、miR-21表达与慢性乙型肝炎病情进展的关联研究[J].现代医药卫生,2025,41(10):2366-2370,5.基金项目
河南省医学科技攻关项目(LHGJ20190120206). （LHGJ20190120206）
