医药导报2025，Vol.44Issue(11)：1814-1820,7.DOI:10.3870/j.issn.1004-0781.2025.11.016
基于扩增受阻突变系统鉴别木香与土木香
Identification of Aucklandia lappa Decne.and Inula helenium L.Based on ARMS Method
摘要
Abstract
Objective To establish a molecular method for the identification of Aucklandia lappa Decne.and its common adulterants,Inula helenium L.,based on tetra-primer amplification refractory mutation system(ARMS).Methods After the analysis and comparison of internal transcribed spacer 2(ITS2)sequence differences between Aucklandia lappa Decne.and Inula helenium L.,single-nucleotide polymorphism(SNP)sites were found to design specific primers for identification,the ARMS method was established to specifically amplify samples from different sources,and identification was carried out according to the size of specific amplification bands.Results The results showed that 170 bp and 271 bp specific bands could be amplified by Aucklandia lappa Decne.and Inula helenium L.,respectively,under the following conditions:annealing temperature 56℃,cycle number 25,primer concentration ratio of internal and external primers 1∶4,agarose gel concentration 1.5%and using 2×TSINGKE Master Mix(blue),the detection limit of adulterates in Aucklandia lappa Decne.and Inula helenium L.mixture sample was 5%.The 23 samples collected from the market were identified,results comparison was the same among ARMS detection,DNA barcoding,and plant classification.Conclusion The ARMS method established in this study can accurately identify Aucklandia lappa Decne.and Inula helenium L.,which has the characteristics of strong specificity and high sensitivity and simple operation,can provide basic research for solving the problem of admixture identification and technical support for the quality control of Aucklandia lappa Decne.medicinal materials.关键词
木香/土木香/扩增受阻突变系统/分子鉴定/掺伪鉴别Key words
Aucklandia lappa Decne./Inula helenium L./Amplification refractory mutation system/Molecular identification/Admixture identification分类
医药卫生引用本文复制引用
赵雄峰,陈小艳,覃桂,刘红,汪波..基于扩增受阻突变系统鉴别木香与土木香[J].医药导报,2025,44(11):1814-1820,7.基金项目
湖北省青年拔尖人才培养计划 （）
湖北中药质控标准物质研制及技术平台的建立(2020ACA007) （2020ACA007）
中国食品药品检定研究院项目(TCM2021-YJ11). （TCM2021-YJ11）
