中国药科大学学报2025，Vol.56Issue(5)：557-565,9.DOI:10.11665/j.issn.1000-5048.2025030302
基于CRISPR/Cas9系统的原位生物成像方法进展
Progress of in situ bioimaging methods based on CRISPR/Cas9 system
摘要
Abstract
In situ bioimaging is a powerful tool for directly observing the localization,expression,and interactions of nucleic acids or protein targets within cells,providing essential insights into cell function and disease mechanisms.In recent years,the CRISPR/Cas9 system,a revolutionary gene-editing tool,has been applied to develop efficient in situ imaging techniques.This paper reviews recent CRISPR/Cas9-based imaging methods utilizing Cas9 protein,engineered single-guide RNA(sgRNA),and coupled fluorescent tags,and compares their application in living and fixed cells.It focuses on the specificity,signal amplification efficiency,and multi-modal imaging capabilities of these methods,with further discussion based on current research,aiming to offer a comprehensive overview of CRISPR/Cas9-based in situ bioimaging techniques,with some valuable reference and guidance for research in related fields.关键词
CRISPR/Cas9/工程化sgRNA/原位成像/荧光标签Key words
CRISPR/Cas9/engineered single-guide RNA/in situ imaging/fluorescence labeling分类
生物科学引用本文复制引用
张惟杰,尹王文康,冯家洛,沈玥,赵羿,王琛,邹秉杰,宋沁馨..基于CRISPR/Cas9系统的原位生物成像方法进展[J].中国药科大学学报,2025,56(5):557-565,9.基金项目
This study was supported by the National Natural Science Foundation of China(No.82173780,No.82404581) （No.82173780,No.82404581）
Jiangsu Provincial Key Research and Development Program for Social Development(BE2023843) （BE2023843）
the Natural Science Foundation of Jiangsu Province(BK20241591) （BK20241591）
and the National College Students Innovation and Entrepreneurship Training Program(No.3322400176) 国家自然科学基金项目(No.82173780,No.82404581) （No.3322400176）
江苏省重点研发计划社会发展项目(BE2023843) （BE2023843）
江苏省自然科学基金项目(BK20241591) （BK20241591）
国家大学生创新创业训练计划项目(No.3322400176) （No.3322400176）
