中国药科大学学报2025，Vol.56Issue(5)：566-571,6.DOI:10.11665/j.issn.1000-5048.2025032001
黄芪显微组织化学定位研究与应用
Research on microscopic histochemical localization of Astragalus membranaceus and its application
摘要
Abstract
This study compared the chemical composition differences among various root tissues of Astragalus membranaceus through optimization of histochemical localization methods,thereby providing some reference for targeted extraction and enrichment of bioactive components.The chloral hydrate permeabilization technique was enhanced with sodium nitrite-aluminum nitrate-sodium hydroxide reagent for flavonoid staining.Comparative analyses of total saponins,total flavonoids,and astragaloside Ⅳ content in xylem,phloem,and periderm tissues were performed using ultraviolet-visible spectroscopy(UV-Vis)and high-performance liquid chromatography(HPLC).By optimizing staining protocols,interference from natural pigmentation was successfully eliminated,thereby enhancing the visualization of tissue-specific chemical distribution.Quantitative analysis revealed that the phloem contained the highest total saponins(6.92%),while the periderm exhibited peak total flavonoids(0.876%)and exclusive enrichment of astragaloside Ⅳ(0.850%).The refined histochemical localization method enables precise characterization of phytochemical distribution across root tissues,which can offer guidance for selective extraction and enrichment strategies,and comprehensively promote the utilization and development of Astragalus membranaceus resources.关键词
黄芪/显微组织化学定位/皂苷/黄酮/黄芪甲苷Key words
Astragalus membranaceus/histochemical localization/saponin/flavonoid/astragaloside Ⅳ分类
医药卫生引用本文复制引用
黄雍,史胜朝,张剑,张朝凤..黄芪显微组织化学定位研究与应用[J].中国药科大学学报,2025,56(5):566-571,6.基金项目
国家自然科学基金项目(No.82374027) This study was supported by the National Natural Science Foundation of China(No.82374027) （No.82374027）
