中国药科大学学报2025，Vol.56Issue(5)：583-591,9.DOI:10.11665/j.issn.1000-5048.2025031101
NSUN2通过介导ARMC9的m5C修饰促进胃癌细胞的增殖、转移和侵袭
NSUN2 promotes proliferation,migration,and invasion of gastric cancer cells by mediating m5C modification of ARMC9
摘要
Abstract
To investigate the impact and underlying mechanism of NOP2/Sun RNA methyltransferase 2(NSUN2)on gastric cancer progression,TCGA database was used and revealed a significant upregulation of NSUN2 expression in gastric cancer tissues.Western blot analysis revealed that NSUN2 was upregulated in gastric cancer cells compared with gastric mucosal epithelial cells.Colony formation assays demonstrated an enhanced colony-forming capacity in NSUN2-overexpressing cells.Furthermore,Transwell assays showed a marked increase in cell migration and invasion upon high NSUN2 expression.Moreover,TCGA database analysis suggested ARMC9 as a potential downstream target of NSUN2.Subsequently,MeRIP-qPCR analysis revealed that NSUN2 overexpression could increase m5C modification of ARMC9 mRNA,and reduce its degradation rate,thus enhancing protein expression.Additionally,ARMC9 overexpression augmented cellular colony formation and migratory and invasive capabilities.These findings indicate that NSUN2 promotes gastric cancer progression by elevating m5C modification of ARMC9 mRNA,increasing its stability and enhancing its expression,therefore,NSUN2 and ARMC9 may serve as potential therapeutic targets for gastric cancer.关键词
5-甲基胞嘧啶/甲基转移酶/NOP2/Sun RNA甲基转移酶2/犰狳重复序列9/胃癌/肿瘤标志物Key words
m5C/methyltransferase/NOP2/Sun RNA methyltransferase 2/armadillo repeat containing 9/gastric cancer/tumor marker分类
医药卫生引用本文复制引用
李越,陈栋,王瑾,彭怡,张远琦,杨芬,王学军..NSUN2通过介导ARMC9的m5C修饰促进胃癌细胞的增殖、转移和侵袭[J].中国药科大学学报,2025,56(5):583-591,9.基金项目
国家自然科学基金项目(No.32270649) This study was supported by the National Natural Science Foundation of China(No.32270649) （No.32270649）
