中国药科大学学报2025，Vol.56Issue(5)：592-600,9.DOI:10.11665/j.issn.1000-5048.2025032601
豆蔻明通过mTOR-ROS轴抑制肺成纤维细胞衰老的机制
Mechanism of cardamonin in inhibiting pulmonary fibroblast senescence through the mTOR-ROS axis
摘要
Abstract
Cardamonin,a natural flavonoid compound,exhibits potential anti-aging properties,yet its precise mechanisms still remain unclear.In this study,we established cellular senescence models using bleomycin and H2O2 and treated the cells with varying concentrations of cardamonin to investigate its anti-senescence effects and underlying mechanisms.Senescence-associated β-galactosidase(SA-β-gal)staining was employed to assess senescent phenotypes,while immunofluorescence was used to detect DNA damage levels.Intracellular reactive oxygen species(ROS)levels were measured using the DCFH-DA probe,and Western blot was performed to analyze the expression of p53,p21,collagen,α-smooth muscle actin(α-SMA),mechanistic target of rapamycin(mTOR),and p-mTOR.To further validate the mechanistic target,MHY1485 was utilized to activate the mTOR pathway and evaluate its regulatory impact on senescence phenotypes.The results demonstrated that cardamonin significantly alleviated bleomycin-and H2O2-induced cellular senescence.Mechanistic studies revealed that cardamonin reduced ROS accumulation and suppressed mTOR phosphorylation.Notably,MHY1485-mediated activation of the mTOR pathway reversed senescence and fibrotic phenotypes,providing reciprocal validation of the target mechanism.In conclusion,cardamonin mitigates cellular senescence by targeting the mTOR-ROS axis,offering a promising therapeutic strategy for anti-aging interventions and the treatment of idiopathic pulmonary fibrosis(IPF).关键词
豆蔻明/衰老/细胞衰老/成纤维细胞/mTOR信号通路Key words
cardamonin/senescence/cellular senescence/fibroblasts/mTOR signaling pathway分类
医药卫生引用本文复制引用
刘颖,李慧星,邵琦,张耀帅,孙磊..豆蔻明通过mTOR-ROS轴抑制肺成纤维细胞衰老的机制[J].中国药科大学学报,2025,56(5):592-600,9.基金项目
国家自然科学基金项目(No.82273934 （）
No.82473928) This study was supported by the National Natural Science Foundation of China(No.82273934 （）
No.82473928) （）
