中国烟草学报2025，Vol.31Issue(5)：48-59,12.DOI:10.16472/j.chinatobacco.2024.T0270
NtWo基因对烟草表皮毛发育的影响研究
Study on the effect of NtWo gene on epidermal hair development in tobacco
摘要
Abstract
[Objective]This study aims to explore the effect of the NtWo gene on epidermal hair development in tobacco.[Methods]Based on homologous cloning,sequence characteristics,and tissue specific expression pattern analysis of NtWo gene,knockout and overexpression materials of the gene were generated.Glandular hair phenotypes were observed,and changes in the expression levels of genes related to glandular hair formation in the knockout and overexpression materials were analyzed.[Results](1)Two homologous NtWo genes,NtWo-1 and NtWo-2,were identified in tobacco,with coding sequences of 2052 bp and 2121 bp,respectively.The encoded proteins contained HD-LZ,START,and SAD domains,belonging to the HD-ZIP IV subfamily.(2)Subcellular localization results showed that NtWo protein was localized in the nucleus.(3)qRT-PCR analysis showed that both NtWo-1 and NtWo-2 were most highly expressed in epidermal hairs,and NtWo-1 expression was higher than NtWo-2 in all tissues except roots.(4)Overexpression of NtWo-1 significantly increased the density of long-stalked glandular hairs on tobacco leaves,while knockout of the NtWo gene significantly reduced the density of long-stalked glandular hairs.The densities of short-stalked glandular hairs and protective hairs did not change significantly in either overexpression or knockout plants.(5)qRT-PCR results showed that NtWo significantly affected the expression of glandular hair formation-related genes,including NtCycB2,NtHD8,NtHD9,NtHD12,NtMIXTA,NtWox3b,NtLFS,and NtBRC2a.[Conclusion]The NtWo gene positively regulates the formation of long-stalked glandular hairs in tobacco.关键词
烟草/HD-ZIP IV/NtWo/基因编辑/腺毛密度Key words
tobacco/HD-ZIP IV/NtWo/genomic editing/glandular trichomes density引用本文复制引用
高嘉宁,王召军,季盈彤,曹依鸣,崔红..NtWo基因对烟草表皮毛发育的影响研究[J].中国烟草学报,2025,31(5):48-59,12.基金项目
中国烟草总公司重大专项项目"烤烟叶面化学成分的分子调控和定向改良"(110202101005(JY-05)) （110202101005(JY-05）
中国烟草总公司重大科技项目"湘烟7号病毒病抗性及叶面腺毛致香物的聚合定向改良"(110202201010(JY-10)) （110202201010(JY-10）
